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As such, rapid and reliable methods to identify emetic strains appear to be clinically relevant. In this study, intact cell matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) was used to differentiate emetic and non\u2010emetic bacilli. The phyloproteomic clustering of 34 <jats:italic>B. cereus<\/jats:italic> emetic and 88 non\u2010emetic isolates classified as <jats:italic>B. cereus<\/jats:italic>, <jats:italic>Bacillus thuringiensis<\/jats:italic>, <jats:italic>Bacillus weihenstephanensis<\/jats:italic>, and <jats:italic>Bacillus mycoides<\/jats:italic>, showed (i) a clear separation of both groups at a similarity level of 43%, and (ii) a high relatedness among the emetic isolates (similarity of 78%). Specifically, 83 mass peak classes were recognized in the spectral window range between <jats:italic>m\/z<\/jats:italic> 4000 and 12\u00a0000 that were tentatively assigned to 41 protein variants based on a bioinformatic approach. Mass variation between the emetic and the non\u2010emetic subsets was recorded for 27 of them, including ten ribosomal subunit proteins, for which inter\u2010strain polymorphism was confirmed by gene sequencing. Additional peaks were assigned to other proteins such as small acid soluble proteins, cold shock proteins and hypothetical proteins, e.g., carbohydrate kinase. Moreover, the results were supported by in silico analysis of the biomarkers in 259 members of <jats:italic>B. cereus<\/jats:italic> group, including <jats:italic>Bacillus anthracis<\/jats:italic>, based on their whole\u2010genome sequences. In conclusion, the proteomic profiling by MALDI\u2010TOF MS is a promising and rapid method for pre\u2010screening <jats:italic>B. cereus<\/jats:italic> to identify medically relevant isolates and for epidemiologic purposes.<\/jats:p>","DOI":"10.1002\/elps.201500308","type":"journal-article","created":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T22:59:32Z","timestamp":1463698772000},"page":"2235-2247","source":"Crossref","is-referenced-by-count":21,"title":["MALDI\u2010TOF MS portrait of emetic and non\u2010emetic <i>Bacillus cereus<\/i> group members"],"prefix":"10.1002","volume":"37","author":[{"given":"Krzysztof","family":"Fiedoruk","sequence":"first","affiliation":[{"name":"Department of Microbiology Medical University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Daniluk","sequence":"additional","affiliation":[{"name":"Department of Microbiology Medical University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Fiodor","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Drewicka","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Buczynska","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Leszczynska","sequence":"additional","affiliation":[{"name":"Department of Microbiology Medical University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis Ken","family":"Bideshi","sequence":"additional","affiliation":[{"name":"Department of Natural and Mathematical Science California Baptist University  Riverside USA"},{"name":"Department of Entomology University of California  Riverside USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela","family":"Swiecicka","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Bialystok  Bialystok Poland"},{"name":"Laboratory of Applied Microbiology University of Bialystok  Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583150801942334"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2008.02.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.185-189.2001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200400055"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-3-1031"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-4-1373"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2013.04.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.030627-0"},{"key":"e_1_2_7_10_1","first-page":"303","volume":"29","author":"Rasko D. 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