{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T11:20:27Z","timestamp":1775042427434,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T00:00:00Z","timestamp":1445817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Scientific Instrument and Equipment Development Project, China","award":["2011YQ03012404"],"award-info":[{"award-number":["2011YQ03012404"]}]},{"name":"State Project for Essential Drug Research and Development, China","award":["2013ZX09507005001"],"award-info":[{"award-number":["2013ZX09507005001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Clenbuterol (CL), as a feed additive, has been banned in many countries due to its potential threat to human health. In detection of CL, a fast, low\u2010cost technique with high accuracy and specificity would be ideal for its administrative on\u2010field inspections. Among the attempts to pursue a reliable detection tool of CL, a technique that combines surface enhanced Raman spectroscopy (SERS) and immunoassay, is close to meet the requirements as above. However, multiple steps of interactions between CL analyte, antibody, and antigen are involved in this method, and under conventional setup, the operation of SERS\/immunoassay were unwieldy. In this paper, to facilitate a more manageable sample manipulation for SERS\u2010immunoassay measurement, a 3D paper chip was suggested. A switch\u2010on\u2010chip multilayered (abbreviated as SoCM\u2010) microfluidic paper\u2010based analysis device (\u03bcPad) was fabricated to provide operators with manual switches on the interactions between different microfluids. Besides, on a detection slip we made on the main body of our SoCM\u2010\u03bcPad, antigen was anchored in pattern. With this architecture, multistep interactions between the CL analyte in swine hair extract and the SERS probe\u2010modified antibody and antigen, were managed for on\u2010chip SERS\u2010immunoassay detection. This would be very attractive for fast, cheap, accurate, and on\u2010site specific detection of CL from real samples.<\/jats:p>","DOI":"10.1002\/elps.201500324","type":"journal-article","created":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T08:34:26Z","timestamp":1442997266000},"page":"418-424","source":"Crossref","is-referenced-by-count":19,"title":["Manual\u2010slide\u2010engaged paper chip for parallel SERS\u2010immunoassay measurement of clenbuterol from swine hair"],"prefix":"10.1002","volume":"37","author":[{"given":"Tingting","family":"Zheng","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Science and Technology Dalian University of Technology  Dalian P. R. China"},{"name":"State Key Laboratory of Fine Chemicals, Department of Chemical Engineering Dalian University of Technology  Dalian P. R. 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