{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:34:38Z","timestamp":1775064878853,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["13-17224S, 15-01948S"],"award-info":[{"award-number":["13-17224S, 15-01948S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Czech Academy of Sciences","award":["RVO61388963"],"award-info":[{"award-number":["RVO61388963"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Affinity capillary electrophoresis (ACE) has been applied to estimation of apparent binding constant of complexes of (<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>)\u2010enantiomers of selected acyclic nucleoside phosphonates (ANPs) with chiral selector \u03b2\u2010cyclodextrin (\u03b2CD) in aqueous alkaline medium. The noncovalent interactions of five pairs of (<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>)\u2010enantiomers of ANPs\u2010based antiviral drugs and their derivatives with \u03b2CD were investigated in the background electrolyte (BGE) composed of 35 or 50 mM sodium tetraborate, pH 10.0, and containing variable concentration (0\u201325 mM) of \u03b2CD. The apparent binding constants of the complexes of (<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>)\u2010enantiomers of ANPs with \u03b2CD were estimated from the dependence of effective electrophoretic mobilities of (<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>)\u2010enantiomers of ANPs (measured simultaneously by ACE at constant reference temperature 25\u00b0C inside the capillary) on the concentration of \u03b2CD in the BGE using different nonlinear and linear calculation methodologies. Nonlinear regression analysis provided more precise and accurate values of the binding constants and a higher correlation coefficient as compared to the regression analysis of the three linearized plots of the effective mobility dependence on \u03b2CD concentration in the BGE. The complexes of (<jats:italic>R<\/jats:italic>,<jats:italic>S<\/jats:italic>)\u2010enantiomers of ANPs with \u03b2CD have been found to be relatively weak \u2013 their apparent binding constants determined by the nonlinear regression analysis were in the range 13.3\u201346.4 L\/mol whereas the values from the linearized plots spanned the interval 12.3\u201355.2 L\/mol.<\/jats:p>","DOI":"10.1002\/elps.201500337","type":"journal-article","created":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T18:54:41Z","timestamp":1443725681000},"page":"239-247","source":"Crossref","is-referenced-by-count":40,"title":["Estimation of apparent binding constant of complexes of selected acyclic nucleoside phosphonates with \u03b2\u2010cyclodextrin by affinity capillary electrophoresis"],"prefix":"10.1002","volume":"37","author":[{"given":"Veronika","family":"\u0160ol\u00ednov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry, v.v.i The Czech Academy of 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