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Therefore, establishing which isolation method is best at removing potential inhibitors may help overcome some of the problems analysts encounter by providing useful information in the determination of the optimal approach for any given sample. Direct analysis in real time (DART) mass spectrometry was used in this study to investigate the ability of different extraction methods to remove PCR inhibitors. Methods investigated included both liquid\/liquid (phenol\u2013chloroform) and solid phase based robotic procedures, (PrepFiler\u2122 and EZ1 chemistries). Following extraction, samples were analyzed by DART in order to determine the level of remaining inhibitors and then quantified and amplified to determine the effect any remaining inhibitor had on the overall results. The data suggests that organic extraction methods result in detrimental amounts of phenol carryover while automated methods may produce carry\u2010over of bile salts and other chemicals that preferentially bind the solid phase matrix. Both of these effects can have a negative impact in downstream sample processing and genotyping by PCR.<\/jats:p>","DOI":"10.1002\/elps.201500480","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T19:24:43Z","timestamp":1459365883000},"page":"2807-2816","source":"Crossref","is-referenced-by-count":8,"title":["The use of direct analysis in real time (DART) to assess the levels of inhibitors co\u2010extracted with DNA and the associated impact in quantification and amplification"],"prefix":"10.1002","volume":"37","author":[{"given":"Lilliana I.","family":"Moreno","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Florida International University  Miami FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce R.","family":"McCord","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Florida International University  Miami FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,19]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"14","volume":"52","author":"Ivanov P.","year":"2009","journal-title":"Sud. 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