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The optimum extraction parameters were a mixture of 250 \u03bcL dichloromethane (extract solvent) and 1250 \u03bcL ACN (dispersive solvent) in 500 \u03bcL of porcine blood reached to pH\u00a06.80. After shaking and centrifugation, the upper phase was transferred in a glass tube and evaporated under N<jats:sub>2<\/jats:sub> steam. The residue was resuspended into 50 \u03bcL of water\u2013ACN (70:30, v\/v) and determined by CE method with DAD, under optimum separation conditions. Consequently, a tenfold enrichment factor can potentially be reached with the pretreatment, taking into account the relationship between initial sample volume and final extract volume. Optimum separation conditions were as follows: BGE solution containing equal amounts of sodium borate (Na<jats:sub>2<\/jats:sub>B<jats:sub>4<\/jats:sub>O<jats:sub>7<\/jats:sub>) and di\u2010sodium hydrogen phosphate (Na<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub>) with a final concentration of 23 mmol\/L containing 0.2% of poly (diallyldimethylammonium chloride) and adjusted to pH 7.80. Separation was performed applying a negative potential of 25 kV, the cartridge was maintained at 25.0\u00b0C and the electropherograms were recorded at 275 nm during 4 min. The hydrodynamic injection was performed in the cathode by applying a pressure of 50 mbar for 10 s.<\/jats:p>","DOI":"10.1002\/elps.201600103","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T00:13:12Z","timestamp":1467677592000},"page":"2670-2677","source":"Crossref","is-referenced-by-count":10,"title":["Dispersive liquid\u2013liquid microextraction of quinolones in porcine blood: Optimization of extraction procedure and CE separation using experimental design"],"prefix":"10.1002","volume":"37","author":[{"given":"Luciana","family":"Vera\u2013Candioti","sequence":"first","affiliation":[{"name":"Universidad Nacional del Litoral, CONICET, FBCB, Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ) Ciudad Universitaria  Santa Fe Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla M.","family":"Teglia","sequence":"additional","affiliation":[{"name":"Universidad Nacional del Litoral, CONICET, FBCB, Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ) Ciudad Universitaria  Santa Fe Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda S.","family":"C\u00e1mara","sequence":"additional","affiliation":[{"name":"UNL, FBCB, Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ) C\u00e1tedra de Qu\u00edmica Anal\u00edtica I  Santa Fe Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,28]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"229","volume-title":"Food Additives","author":"Appiah Ofori J.","year":"2012"},{"key":"e_1_2_8_3_1","first-page":"127","volume-title":"Advances in Meat Research","author":"Dill C. 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