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The extravasation of tumor cells is a critical event in tumor metastasis. However, the process and mechanism that underlie tumor cell extravasation remain unclear, which restricts the examination of many tumor processes and presents a formidable hurdle to drug development. To explore the initial steps by which lung tumor cells interact with the brain microvascular wall in the course of extravasation, we present a simple, inexpensive, and time\u2010saving microfluidic device to mimic the inflammatory brain microvascular microenvironment and to investigate both the biochemical and mechanical causes of lung tumor cell rolling and adhesion on inflammatory endothelium to analyze the synergistic effects on tumor extravasation under fluidic shear stress conditions. Under microvascular inflammation induced by tumor necrosis factor \u03b1, the lung tumor cells (A549 cells) displayed significant adhesion activity. In addition, we found that this situation could be reversed by administration of Rho\/Rho\u2010associated protein serine\/threonine kinase (ROCK) inhibitor (Y27632). 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