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Such information\u00a0can provide critical context to evidence and can help to prioritize downstream DNA analyses. Previous proteome studies compiled panels of \u201ccandidate biomarkers\u201d specific to each of five body fluids (<jats:italic>i.e<\/jats:italic>., peripheral blood, vaginal\/menstrual fluid, seminal fluid, urine, and saliva). Here, a multiplex quadrupole time\u2010of\u2010flight mass spectrometry assay has been developed in order to verify the tissue\/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a sample population of 50 humans. Single\u2010source samples of these human body fluids were accurately identified by the detection of one or more high\u2010specificity biomarkers. Recovery of body fluid samples from a variety of substrates did not impede accurate characterization and, of the potential inhibitors assayed, only chewing tobacco juice appeared to preclude the identification of a target body fluid. Using a series of 2\u2010component mixtures of human body fluids, the multiplex assay accurately identified both components in a single\u2010pass. Only in the case of saliva and peripheral blood did matrix effects appear to impede the detection of salivary proteins.<\/jats:p>","DOI":"10.1002\/elps.201600352","type":"journal-article","created":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T04:22:45Z","timestamp":1481516565000},"page":"833-845","source":"Crossref","is-referenced-by-count":49,"title":["Verification of protein biomarker specificity for the identification of biological stains by quadrupole time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"38","author":[{"given":"Kevin M.","family":"Legg","sequence":"first","affiliation":[{"name":"Department of Biological Sciences University of Denver  Denver Colorado USA"},{"name":"The Center for Forensic Science Research and Education  Willow Grove PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Powell","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichole","family":"Reisdorph","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rick","family":"Reisdorph","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip B.","family":"Danielson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Denver  Denver Colorado USA"},{"name":"The Center for Forensic Science Research and Education  Willow Grove PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.02.013"},{"key":"e_1_2_8_3_1","volume-title":"Sourcebook in Forensic Serol. 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