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The nano\u2010DEP device was fabricated by bonding two SU\u20108 covered electrodes patterned using photolithography. The bottom electrode contains a 200 \u00d7 200 \u03bcm<jats:sup>2<\/jats:sup> active region on a randomly distributed NEA and the top electrode contains a microfluidic channel in SU\u20108 spin\u2010coated on ITO to guide the flow of the virus solution. The real\u2010time impedance change was measured during DEP capture and validated with fluorescence microscopy measurements. The NEA was able to capture virus particles with a rather low AC voltage (\u223c8.0 V peak\u2010to\u2010peak) at 1.0 kHz frequency as the particles were passed through the fluidic channel at high flow velocities (up to 8.0 mm\/s). A concentration detection limit as low as \u223c2.58 \u00d7 10<jats:sup>3<\/jats:sup> particles\/mL was obtained via impedance measurements after only 54 sec of DEP capture. At the low AC frequencies (50.0 Hz or less), the high electric field at the exposed VACNF tips induced electroporation of the DEP\u2010captured virus particles, which was validated by fluorescence emission from the dyes staining lipophilic membrane and internal nucleic acid, respectively. This study suggests the possibility of integration of a fully functional electronic device for rapid, reversible and label\u2010free capture and detection of pathogenic viruses, with a potential of generating electroporation to the captured the virus particles for further biochemical study.<\/jats:p>","DOI":"10.1002\/elps.201600436","type":"journal-article","created":{"date-parts":[[2017,2,17]],"date-time":"2017-02-17T01:22:42Z","timestamp":1487294562000},"page":"1515-1525","source":"Crossref","is-referenced-by-count":23,"title":["AC dielectrophoretic manipulation and electroporation of vaccinia virus using carbon nanoelectrode arrays"],"prefix":"10.1002","volume":"38","author":[{"given":"Foram Ranjeet","family":"Madiyar","sequence":"first","affiliation":[{"name":"Department of Physical Sciences Embry\u2010Riddle Aeronautical University  Daytona Beach FL USA"},{"name":"Department of Chemistry Kansas State University  Manhattan KS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherry L.","family":"Haller","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Medical Branch  Galveston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omer","family":"Farooq","sequence":"additional","affiliation":[{"name":"Department of Physical Sciences Embry\u2010Riddle Aeronautical University  Daytona Beach FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Rothenburg","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine the University of California at Davis  Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Culbertson","sequence":"additional","affiliation":[{"name":"Department of Chemistry Kansas State University  Manhattan KS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry Kansas State University  Manhattan KS USA"},{"name":"College of Chemistry and Chemical Engineering Hubei Normal University  Huangshi P. 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