{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T14:04:42Z","timestamp":1778681082189,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,1,6]],"date-time":"2018-01-06T00:00:00Z","timestamp":1515196800000},"content-version":"am","delay-in-days":337,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,2,3]],"date-time":"2017-02-03T00:00:00Z","timestamp":1486080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000171","name":"Division of Human Resource Development","doi-asserted-by":"publisher","award":["0934146, 1547723"],"award-info":[{"award-number":["0934146, 1547723"]}],"id":[{"id":"10.13039\/100000171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000149","name":"Division of Engineering Education and Centers","doi-asserted-by":"publisher","award":["0812348"],"award-info":[{"award-number":["0812348"]}],"id":[{"id":"10.13039\/100000149","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000089","name":"Office of International Science and Engineering","doi-asserted-by":"publisher","award":["1448166"],"award-info":[{"award-number":["1448166"]}],"id":[{"id":"10.13039\/100000089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>In this paper, a microfluidic thread\u2010based analytical device (\u03bcTAD) to assess the activity of acetylcholinesterase (AChE) via colorimetric analylsis is described. Fabrication of the device consists of two platforms, both with a nylon thread trifurcated into three channels terminating at open analysis sites at the end of the thread. 5,5\u2019\u2010Dithiobis\u2010(2\u2010nitrobenzoic acid) (DTNB) was spotted and dried on the analysis sites. Acetylthiocholine iodide (ATC) (or cysteine, Cys) is transported through an inlet channel of the nylon thread by capillary action due to the hydrophilic nature of nylon. AChE is transported through the other inlet channel and mixes with the ATC (or Cys) as they travel up to the analysis sites. As the solution reaches the analysis sites, an intense yellow color change occurs indicating the reaction of the thiol with DTNB to produce the yellow anion TNB<jats:sup>2\u2212<\/jats:sup>. The sites are then dried, scanned, yielding a linear range of inverse yellow mean intensity versus substrate concentration. An IC<jats:sub>50<\/jats:sub> value (1.74 nM) with a known inhibitor, neostigmine bromide (NB), is obtained on the device. The multiplex design enables triplicate data collection in a device that is easy to use. \u03bcTADs have great potential to be employed in a myriad of tests including point\u2010of\u2010care diagnostic devices for resource\u2010challenged settings.<\/jats:p>","DOI":"10.1002\/elps.201600476","type":"journal-article","created":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T03:52:41Z","timestamp":1483674761000},"page":"996-1001","source":"Crossref","is-referenced-by-count":28,"title":["Thread\u2010based microfluidic chips as a platform to assess acetylcholinesterase activity"],"prefix":"10.1002","volume":"38","author":[{"given":"Ariana","family":"Gonzalez","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University, Los Angeles  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Gaines","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University, Los Angeles  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank A.","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University, Los Angeles  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.12.307"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200603817"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.14.240"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202837s"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2AN36219H"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/la501212b"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC00465A"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac403026c"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA07112C"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY00466G"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.11.025"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4005(90)80209-I"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2015.08.030"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC01392E"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50169h"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201400421"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201400091"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac503968p"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2014.03.022"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac401786h"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2014.12.042"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac4008623"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.01.054"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2014.07.070"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/am9006148"},{"key":"e_1_2_8_27_1","first-page":"051501\u20101","volume":"7","author":"Nilghaz A.","year":"2013","journal-title":"Biomicrofliudics"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1lc20336c"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/am1002266"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac301082d"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201600029"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2982213"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.741"},{"key":"e_1_2_8_34_1","author":"Liu C.","year":"2017","journal-title":"Electrophoresis"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201600476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201600476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.201600476","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/elps.201600476","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201600476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:23:39Z","timestamp":1760527419000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201600476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,3]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1002\/elps.201600476"],"URL":"https:\/\/doi.org\/10.1002\/elps.201600476","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,3]]}}}