{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:39:58Z","timestamp":1773754798243,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Fatty acids (FAs) have been selectively derivatized with a fluorescent tag, 6\u2010aminoquinoline (6AQ), which yielded fluorescent FA\u20106AQ derivatives that have excitation (\u03bb<jats:sub>exc<\/jats:sub> = 270 nm) and emission (\u03bb<jats:sub>emi<\/jats:sub> = 495 nm) wavelengths that are farther apart. This precolumn derivatization is characterized by its simplicity occurring at room temperature between the carboxylic acid group of the FA and the amino group of 6AQ in the presence of a nonaqueous soluble carbodiimide coupling agent such as the <jats:italic>N,N\u00b4<\/jats:italic>\u2010dicyclohexylcarbodiimide. The FAs extracts are readily derivatized in chloroform and can be analyzed without any further sample cleanup that minimizes sample loss. The FA\u20106AQ derivatives derived from standard FAs as well as from extracted FAs from food samples were separated by reversed phase chromatography on a homemade naphthyl methacrylate monolithic (NMM) column and C4 silica\u2010based column. While the NMM column provided excellent separation for saturated FA\u20106AQ derivatives, the C4 silica column was able to separate simultaneously saturated and unsaturated FA\u20106AQ derivatives. The MNN column permitted the analysis and quantitation of the saturated FA\u20106AQ derivatives extracted from coconut oil. The C4 column provided the selectivity needed to analyze and quantify saturated and unsaturated derivatized with 6AQ and extracted from meat. The limits of detection and quantitation were 5 and 20 nM, respectively, with a linear dynamic range extending from 20 nM to 40 \u03bcM. The 40 \u03bcM upper limit was due to the limited solubility of the FA\u20106AQ derivatives in the diluting mobile phase, which is the initial mobile phase used in gradient runs.<\/jats:p>","DOI":"10.1002\/elps.201600544","type":"journal-article","created":{"date-parts":[[2017,1,28]],"date-time":"2017-01-28T06:52:45Z","timestamp":1485586365000},"page":"1592-1601","source":"Crossref","is-referenced-by-count":7,"title":["Selective precolumn derivatization of fatty acids with the fluorescent tag 6\u2010aminoquinoline and their determination in some food samples by reversed\u2010phase chromatography"],"prefix":"10.1002","volume":"38","author":[{"given":"Murthy","family":"Jonnada","sequence":"first","affiliation":[{"name":"Department of Chemistry Oklahoma State University  Stillwater OK USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe Davila","family":"El Rassi","sequence":"additional","affiliation":[{"name":"Robert M. 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