{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:06:24Z","timestamp":1790492784224,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T00:00:00Z","timestamp":1487635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["97101"],"award-info":[{"award-number":["97101"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Multiple myeloma (MM) is an immedicable malignancy of the human plasma cells producing abnormal antibodies (also referred to as paraproteins) leading to kidney problems and hyperviscosity syndrome. In this paper, we report on the N\u2010glycosylation analysis of paraproteins from total human serum as well as the fragment crystallizable region (F<jats:sub>c<\/jats:sub>) and fragment antigen binding (F<jats:sub>ab<\/jats:sub>) \u03ba\/\u03bb light chain fractions of papain digested immunoglobulins from multiple myeloma patients. CE\u2010LIF detection was used for the analysis of the N\u2010glycans after endoglycosidase (PNGase F) mediated sugar release and fluorophore labeling (APTS). While characteristic N\u2010glycosylation pattern differences were found between normal control and untreated, treated and remission stage multiple myeloma patient samples at the global serum level, less distinctive changes were observed at the immunoglobulin level. Principal component analysis adequately differentiated the four groups (control and three patient groups) on the basis of total serum N\u2010glycosylation analysis. 12 N\u2010glycan features showed statistically significant differences (<jats:italic>p<\/jats:italic> &lt;0.05) among various stages of the disease in comparison to the control at the serum level, while only six features were identified with similar significance at the immunoglobulin level, including the analysis of the partitioned F<jats:sub>c<\/jats:sub> fragment as well as the F<jats:sub>ab<\/jats:sub>\u03ba and F<jats:sub>ab<\/jats:sub>\u03bb chains.<\/jats:p>","DOI":"10.1002\/elps.201700006","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T02:26:43Z","timestamp":1485224803000},"page":"2115-2123","source":"Crossref","is-referenced-by-count":23,"title":["Capillary electrophoresis analysis of N\u2010glycosylation changes of serum paraproteins in multiple myeloma"],"prefix":"10.1002","volume":"38","author":[{"given":"Zsuzsanna","family":"Kovacs","sequence":"first","affiliation":[{"name":"Horv\u00e1th Csaba Memorial Institute for Bioanalytical Research University of Debrecen  Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Simon","sequence":"additional","affiliation":[{"name":"Horv\u00e1th Csaba Memorial Institute for Bioanalytical Research University of Debrecen  Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Szabo","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  Sunnyvale CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Nagy","sequence":"additional","affiliation":[{"name":"1st Department of Internal Medicine Semmelweis University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laszlo","family":"Varoczy","sequence":"additional","affiliation":[{"name":"Department of Hematology Institute for Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ildiko","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Hematology Institute for Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Csanky","sequence":"additional","affiliation":[{"name":"Semmelweis Hospital  Miskolc Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andras","family":"Guttman","sequence":"additional","affiliation":[{"name":"Horv\u00e1th Csaba Memorial Institute for Bioanalytical Research University of Debrecen  Hungary"},{"name":"MTA\u2010PE Translational Glycomics Research Group University of Pannonia  Veszprem Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.291"},{"key":"e_1_2_8_3_1","first-page":"240281","volume":"2012","author":"Girish G.","year":"2012","journal-title":"ScientificWorld J."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr9001004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09837"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2015.339"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4065\/78.1.21"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1093\/clinchem\/48.9.1437","volume":"48","author":"Katzmann J. 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