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Analytes were first derivatized with 1,1\u2032\u2010thiocarbonyldiimidazole and then samples were purified by chloroform\u2013ACN extraction. Electrophoretic separation was performed using 0.1\u00a0M phosphate with 30\u00a0mM triethanolamine, pH 2, containing 25\u00a0\u03bcM CTAB, 2.5\u00a0\u03bcM SDS, and 2.5% polyethylene glycol 600. Samples were injected into the capillary (with total length 32\u00a0cm and 50\u00a0\u03bcm id) at 2250\u00a0mbar*s and subsequent injection was performed for 30\u00a0s with 0.5\u00a0M K\u041e\u041d. The total analysis time was less than 9\u00a0min, accuracy was 98%, and precision was &lt;2.6%. The LOD was 0.2\u00a0\u03bcM for homocysteine and 0.5\u00a0\u03bcM for cysteine. The use of liquid\u2013liquid extraction allowed the precision and sensitivity of the CE method to be significantly increased. The validated method was applied to determine total cysteine and homocysteine content in human blood plasma and urine samples obtained from healthy volunteers and patients with kidney disorders.<\/jats:p>","DOI":"10.1002\/elps.201700133","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T08:22:37Z","timestamp":1499329357000},"page":"2646-2653","source":"Crossref","is-referenced-by-count":30,"title":["Capillary electrophoresis coupled with chloroform\u2010acetonitrile extraction for rapid and highly selective determination of cysteine and homocysteine levels in human blood plasma and urine"],"prefix":"10.1002","volume":"38","author":[{"given":"Alexander Vladimirovich","family":"Ivanov","sequence":"first","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of General Pathology and Pathophysiology\u201d  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polina Olegovna","family":"Bulgakova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of General Pathology and Pathophysiology\u201d  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward Danielevich","family":"Virus","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of General Pathology and Pathophysiology\u201d  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Petrovna","family":"Kruglova","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Educational Institution of Higher Education I.M. 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