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Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B\u2010subunit (CTB) and unlabeled carbohydrate ligands based on ganglioside GM1\u2010oligosaccharides (GM1os). In an optimized method at pH\u00a04, adsorption of the protein to the capillary walls was prevented by a polybrene\u2010dextran sulfate\u2010polybrene coating. Different concentrations of the ligands were added to the BGE. CTB binding was observed by a mobility shift that could be used for dissociation constant (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) determination. The <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values of two GM1 derivatives differed by close to an order of magnitude (600\u00a0\u00b1\u00a020\u00a0nM and 90\u00a0\u00b1\u00a050\u00a0nM) which was in good agreement with the differences in their reported nanomolar IC<jats:sub>50<\/jats:sub> values of an ELISA\u2010type assay. Moreover, the selectivity of GM1os towards CTB was demonstrated using Influenza hemagglutinin (H5) as a binding competitor. The developed method can be an important platform for preclinical development of drugs targeting pathogen\u2010induced secretory diarrhea.<\/jats:p>","DOI":"10.1002\/elps.201700207","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T04:06:53Z","timestamp":1505362013000},"page":"344-347","source":"Crossref","is-referenced-by-count":12,"title":["Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate\u2010based cholera toxin inhibitors"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2321-2113","authenticated-orcid":false,"given":"Oier","family":"Aizpurua\u2010Olaizola","sequence":"first","affiliation":[{"name":"Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences Utrecht University  Utrecht Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Sastre 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