{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:46:59Z","timestamp":1764784019447,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enantiomeric separation of methadone in the presence of multiple isomer mixtures of sulfated \u03b2\u2010cyclodextrin (S\u2010\u03b2\u2010CD) was studied experimentally with CZE and theoretically using computer simulation. Experiments were performed over many years with several lots of S\u2010\u03b2\u2010CD from the same manufacturer with a specified degree of substitution of 7\u201311. Large differences in the migration patterns were observed between certain lots and it was concluded that the extent of labelling in lots released after a transition time was higher than originally specified. The migration pattern was observed to be associated with (i) the ionic strength increase resulting from using S\u2010\u03b2\u2010CDs with a higher charge state and (ii) differences in buffer composition. Apparent binding constants between methadone and the S\u2010\u03b2\u2010CD and complex mobilities were determined for different lots of S\u2010\u03b2\u2010CD at varying ionic strength using phosphate and 3\u2010morpholino\u20102\u2010hydroxypropanesulfonic acid buffers. The obtained values were used as input for simulations. For a given ionic strength, agreement between predicted and experimentally observed behavior was obtained for different buffers. R\u2010methadone has a stronger interaction with S\u2010\u03b2\u2010CD than S\u2010methadone. For any given configuration there is a distinct S\u2010\u03b2\u2010CD concentration range which results in the cationic migration of S\u2010methadone while the migration direction of R\u2010methadone is reversed. This configuration was demonstrated to be applicable for micropreparative CZE separations.<\/jats:p>","DOI":"10.1002\/elps.201700376","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T07:32:36Z","timestamp":1509521556000},"page":"770-778","source":"Crossref","is-referenced-by-count":14,"title":["Computer simulation and enantioselective capillary electrophoresis to characterize isomer mixtures of sulfated \u03b2\u2010cyclodextrins"],"prefix":"10.1002","volume":"39","author":[{"given":"Saara","family":"Mikkonen","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Laboratory Institute for Infectious Diseases University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitka","family":"Caslavska","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory Institute for Infectious Diseases University of Bern  Bern 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