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The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE\u2010 immobilized microenzyme reactors (IMER) development, human flavin\u2010containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in\u2010line capillary electrophoresis with clozapine as a model substrate. This setup could be used further to establish CE\u2010IMERs of other drug metabolic enzymes in a commercially available liquid based capillary coolant system. The CE\u2010IMER setup was successful, although a subsequent decrease in enzyme activity was observed on repeated runs.<\/jats:p>","DOI":"10.1002\/elps.201700434","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T20:15:47Z","timestamp":1515528947000},"page":"981-988","source":"Crossref","is-referenced-by-count":19,"title":["An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors"],"prefix":"10.1002","volume":"39","author":[{"given":"Pranov","family":"Ramana","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences Pharmaceutical Analysis KU Leuven \u2010 University of Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Schejbal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science Masaryk University  Brno Czech 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