{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:03:35Z","timestamp":1788339815175,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T00:00:00Z","timestamp":1528070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deanship of Scientific Research at King Saud University"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Proteomic methods have great potential to aid our understanding of the functional and pathological roles of adipose tissue. A critical initial step in the proteomic studies is the efficient isolation of proteins before conducting detailed analysis. In this study, three different methods were used for precipitating proteins; we analyzed samples from visceral adipose tissue, subcutaneous adipose tissue, and stromal visceral fraction extracts after chloroform\/methanol, acetone, and trichloroacetic acid precipitation. The proteins recovered after the precipitation steps were examined by 2D\u2010DIGE. Statistical analyses were carried out using simple linear regression analyses and R2 values were calculated for the intra\u2010 and inter\u2010method comparisons. We found that all three precipitation methods provided highly reproducible protein spots that were recovered when run in duplicate using the same method of precipitation, irrespective of whether it was solvent (<jats:italic>R<\/jats:italic>2\u00a0=\u00a00.85\u20130.98) or acid\u2010based (<jats:italic>R<\/jats:italic>2\u00a0=\u00a00.80\u20130.96). A higher variation and poor correlation was noted for the recovered protein spots when comparisons were made between the methods (R2\u00a0=\u00a00.40\u20130.88) and also when the same method was compared between different sample types. In this study, TCA\u2010precipitated samples were enriched in lower molecular mass proteins compared to the samples extracted by solvent\u2010based precipitation methods.<\/jats:p>","DOI":"10.1002\/elps.201800124","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T02:27:13Z","timestamp":1525746433000},"page":"1745-1753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of protein precipitation methods from adipose tissue using difference gel electrophoresis"],"prefix":"10.1002","volume":"39","author":[{"given":"Hicham","family":"Benabdelkamel","sequence":"first","affiliation":[{"name":"Proteomics Resource Unit, Obesity Research Center, College of Medicine King Saud University  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9745-2959","authenticated-orcid":false,"given":"Afshan","family":"Masood","sequence":"additional","affiliation":[{"name":"Proteomics Resource Unit, Obesity Research Center, College of Medicine King Saud University  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim O","family":"Alanazi","sequence":"additional","affiliation":[{"name":"National Centre for Biotechnolgy King Abdulaziz City for Science and Technology (KACST)  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assim A","family":"Alfadda","sequence":"additional","affiliation":[{"name":"Proteomics Resource Unit, Obesity Research Center, College of Medicine King Saud University  Riyadh Kingdom of Saudi Arabia"},{"name":"Department of Medicine College of Medicine King Saud University  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.280.6.E827"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000350"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.05.027"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100355"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2013.07.008"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.14348\/molcells.2017.0073","volume":"40","author":"Alfadda A. 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