{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:53:10Z","timestamp":1760575990085,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Quality & Safety of Aquatic Products Technical Support Team","award":["QS2017003"],"award-info":[{"award-number":["QS2017003"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tetracyclines abuse has frequently occurred in aquaculture against bacteria, rickettsiae, spirochetes, and mycoplasmas. In this study, a high\u2010throughput sample preparation method was developed using 96\u2010well plate solid\u2010phase extraction (<jats:italic>p<\/jats:italic>\u2010SPE) and the extract was analyzed by ultra\u2010performance liquid chromatography tandem mass spectrometry (UPLC\u2010MS\/MS). The experimental conditions were optimized such that the pH is 4, the eluting solvent is methanol (2 mL), and the sorbent is hydrophilic\u2010lipophilic balance (HLB) microsphere. The whole protocol was validated, and it showed that the tetracyclines were linear with correlation coefficients \u2265 0.9990, precision and accuracy (RSD%) in 3.9\u20136.1%, and mean recoveries of 88.6\u2013103.6%. To exhibit the potential of 96\u2010well <jats:italic>p<\/jats:italic>\u2010SPE as a routine tool for inspection and quarantine, fresh aquatic samples were tested, and among which positive samples were observed. This method was demonstrated to be promising for the purification and enrichment of tetracyclines with reduced time and labor, and indeed practically and particularly suitable for widespread tetracyclines analysis.<\/jats:p>","DOI":"10.1002\/elps.201800473","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T02:50:22Z","timestamp":1543978222000},"page":"555-562","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High\u2010throughput 96\u2010well solid\u2010phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis"],"prefix":"10.1002","volume":"40","author":[{"given":"Kang","family":"Chen","sequence":"first","affiliation":[{"name":"The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province Institute of Seafood Zhejiang Gongshang University  Hangzhou Zhejiang Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province Institute of Seafood Zhejiang Gongshang University  Hangzhou Zhejiang Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyan","family":"Li","sequence":"additional","affiliation":[{"name":"Aquatic Products Quality Inspection Center of Zhejiang Province  Hangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Aquatic Products Quality Inspection Center of Zhejiang Province  Hangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province Institute of Seafood Zhejiang Gongshang University  Hangzhou Zhejiang Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9418-0182","authenticated-orcid":false,"given":"Qing","family":"Shen","sequence":"additional","affiliation":[{"name":"The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province Institute of Seafood Zhejiang Gongshang University  Hangzhou Zhejiang Province P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118675014.ch15"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-51.8.632"},{"key":"e_1_2_9_4_1","first-page":"1","author":"Council Regulation (EEC) No 2377","year":"1990","journal-title":"Off. J. Eur. Commun."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.10.029"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.09.011"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2010.959.962"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/09540105.2015.1036357"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-014-7649-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652039109373955"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-010-0428-8"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac800936r"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2013.01.004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01221-3"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0231(20010115)15:1<33::AID-RCM188>3.0.CO;2-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.08.050"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.06.030"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bk-1996-0636"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.04.013"},{"key":"e_1_2_9_20_1","first-page":"1","volume":"4","author":"Li L.","year":"2015","journal-title":"J. Anal. Methods Chem."},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2007.02.007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.09.066"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00282-9"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.07.014"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00725-1"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.01.027"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.10.012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(98)00078-6"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.07.042"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201800473","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201800473","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.201800473","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201800473","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:48:35Z","timestamp":1760543315000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201800473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,13]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/elps.201800473"],"URL":"https:\/\/doi.org\/10.1002\/elps.201800473","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2018,12,13]]},"assertion":[{"value":"2018-11-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}