{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T09:15:03Z","timestamp":1771578903979,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T00:00:00Z","timestamp":1559779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004602","name":"Program for New Century Excellent Talents in University","doi-asserted-by":"publisher","award":["NCET\u201009\u20100434"],"award-info":[{"award-number":["NCET\u201009\u20100434"]}],"id":[{"id":"10.13039\/501100004602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis is highly persistent and displays phenotypic resistance to high concentrations of antimicrobials. Recent reports exhibited that <jats:italic>Mycobacterium tuberculosis<\/jats:italic> biofilm was implicated to its pathogenicity and drug resistance. In this study, there were 47 kinds of differential proteins in the biofilm of <jats:italic>M. tuberculosis<\/jats:italic> H37Rv cells compared with the planktonic bacteria, and 37 proteins were nonredundant and identified by proteomics approach, such as 2DE and LC\u2010MS\/MS. Moreover, six kinds of proteins were identified as HspX, which were conservative and highly expressed in biofilm. Note that 47 differential proteins were divided into seven categories, such as cell wall and cell processes, conserved hypotheticals, intermediary metabolism and respiration, and so on by TUBERCULIST. The Gene Ontology classification results showed that the largest protein group involved in metabolism, binding proteins, and catalytic function accounts for 30% and 57% of all identified proteins, respectively. Moreover, the protein interaction network analyzed by STRING showed that the minority proteins such as RpoA, SucC, Cbs, Tuf, DnaK, and GroeL in the interaction network have high network connectivity. These results implied that the proteins involved in metabolic process and catalytic function and the minority proteins mentioned above may play an important role in <jats:italic>M. tuberculosis<\/jats:italic> biofilm formation. To our knowledge, this is the first report about differential proteins between biofilm and planktonic <jats:italic>M. tuberculosis<\/jats:italic>, which provided the potential antigens for vaccines and target proteins for anti\u2010mycobacterial drugs.<\/jats:p>","DOI":"10.1002\/elps.201900030","type":"journal-article","created":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T09:41:03Z","timestamp":1559122863000},"page":"2736-2746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparative proteomics analysis between biofilm and planktonic cells of <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1002","volume":"40","author":[{"given":"Chao","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Lab for New Drug Research of TCM Research Institute of Tsinghua University in Shenzhen  Shenzhen P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"An","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Luan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses  Yangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5216-3843","authenticated-orcid":false,"given":"Lu","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases First Hospital of Jilin University, College of Veterinary Medicine, Jilin University  Changchun P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(14)70361-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arbr.2016.07.027"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12142"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1211"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2007.12.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.49"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06274.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1049-1059.2006"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500531"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-009-0158-y"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11155.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06304-11"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.12.013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35084083"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014076-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3927-3936.2003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03900.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002511"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444912007330"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-234"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054204"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi702453s"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10969-009-9073-z"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047781"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00917"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000460"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2014.971762"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00143-09"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2012.03.001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05469-11"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00729-0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00931-14"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05455.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(04)00002-3"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2017.07.006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.8.2087"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510580103"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1518-1529.2002"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.025635"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201900030","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201900030","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.201900030","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201900030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T14:24:05Z","timestamp":1760538245000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201900030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,6]]},"references-count":39,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/elps.201900030"],"URL":"https:\/\/doi.org\/10.1002\/elps.201900030","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,6]]},"assertion":[{"value":"2019-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}