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The assay is based on liquid\/liquid extraction of the analytes at alkaline pH from 0.1 mL plasma followed by electrokinetic sample injection of the analytes from the extract across a buffer plug without chiral selector. Separation occurs cationically at normal polarity in a pH 3 phosphate buffer containing 0.16% (w\/v) of highly sulfated \u03b3\u2010cyclodextrin. The developed assay is precise (intra\u2010 and interday RSD &lt; 4% and &lt; 7%, respectively), is capable to determine enantiomer levels of methadone and 2\u2010ethylidene\u20101,5\u2010dimethyl\u20103,3\u2010diphenylpyrrolidine in plasma down to 2.5\u00a0ng\/mL, and was successfully applied to monitor enantiomer drug and metabolite levels in plasma of a pony that was anesthetized with racemic ketamine and isoflurane and received a bolus of racemic methadone and a bolus followed by constant rate infusion of racemic methadone. The data suggest that the assay is well suited for pharmacokinetic purposes.<\/jats:p>","DOI":"10.1002\/elps.201900044","type":"journal-article","created":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T04:10:42Z","timestamp":1553227842000},"page":"1959-1965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2\u2010ethylidene\u20101,5\u2010dimethyl\u20103,3\u2010diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane"],"prefix":"10.1002","volume":"40","author":[{"given":"Regula","family":"Theurillat","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Laboratory Institute for Infectious Diseases University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike A.","family":"Sandbaumh\u00fcter","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory Institute for Infectious Diseases University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Gittel","sequence":"additional","affiliation":[{"name":"Department for Horses University of Leipzig  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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