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An analytical\u2010quality\u2010by\u2010design approach was embraced for method development, method implementation, and method maintenance. CZE provided separation of adenovirus particles from sample matrix components, such as cell debris, residual DNA and proteins. The intermediate precision of the virus particle concentration was 6.9% RSD and the relative bias was 2.3%. In comparison, the CZE method is intended to replace a quantitative polymerase chain reaction method which requires three replicates in three analytical runs to achieve an intermediate precision of 8.1% RSD. Given that, in addition, the time from sampling till reporting results of the CZE method was less than 2 h, whereas quantitative polymerase chain reaction requires 3 days, it follows that the CZE method enables faster processing times in downstream processing.<\/jats:p>","DOI":"10.1002\/elps.201900068","type":"journal-article","created":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T05:37:34Z","timestamp":1554529054000},"page":"2277-2284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Implementation of at\u2010line capillary zone electrophoresis for fast and reliable determination of adenovirus concentrations in vaccine manufacturing"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8001-4538","authenticated-orcid":false,"given":"Ewoud","family":"van Tricht","sequence":"first","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Geurink","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"},{"name":"Faculty of Pharmacy Department of Medicinal Chemistry Division of Analytical Pharmaceutical Chemistry Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Galindo Garre","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martijn","family":"Schenning","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold","family":"Backus","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Germano","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Analytical Development Janssen Vaccines and Prevention  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Govert W.","family":"Somsen","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Amsterdam Division of BioMolecular Analysis Amsterdam Institute of Molecules, Medicines and Systems  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8409-4443","authenticated-orcid":false,"given":"Cari E.","family":"S\u00e4nger \u2013 van de Griend","sequence":"additional","affiliation":[{"name":"Kantisto BV  Baarn The Netherlands"},{"name":"Faculty of Pharmacy Department of Medicinal Chemistry Division of Analytical Pharmaceutical Chemistry Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.537498"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2013.235"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2015.993374"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02696-06"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.15.8263-8271.2003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012199504-1\/50008-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652310793797694"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.281"},{"key":"e_1_2_7_10_1","first-page":"397","volume-title":"Separation Science and Technology","author":"Wood C.","year":"2011"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1997.8.4-453"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00330-9"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00597-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00257-9"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2017.01.013"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00755-X"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/868727"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22325"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.6872"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1093\/clinchem\/20.7.825","volume":"20","author":"Westgard J. 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