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While it has been reported that the <jats:italic>N<\/jats:italic>\u2010glycan moieties of glycoproteins is not affected by the FFPE process, no information is available about the effect of the elapsed time between sampling and fixation on the resulting <jats:italic>N<\/jats:italic>\u2010glycosylation profile. In this study, lung, brain, heart, spleen, liver, kidney, and intestine mouse tissue specimens were used for <jats:italic>N<\/jats:italic>\u2010glycan profiling analysis and the elapsed sampling time effect was investigated with the lung tissue. <jats:italic>N<\/jats:italic>\u2010glycan extraction from the tissue samples was performed by glycoprotein retrieval from the FFPE specimens using radioimmunoprecipitation assay (RIPA) buffer followed PNGase F digestion. The released oligosaccharides were fluorophore labeled and analyzed by capillary electrophoresis\u2010laser induced fluorescent detection (CE\u2010LIF). <jats:italic>N<\/jats:italic>\u2010glycosylation profiles of freshly collected lung\u2010tissue samples (zero time point), as well as 1 and 2 h after sampling were compared by carbohydrate profiling and exoglycosidase treatment based deep glycomic analysis. It was found that up to two hours of room temperature storage of tissue specimens apparently did not cause changes in the <jats:italic>N<\/jats:italic>\u2010glycosylation profiles of complex carbohydrates, but resulted in considerable decrease in the amount of linear glucose oligomers and high mannose type glycans present in the samples.<\/jats:p>","DOI":"10.1002\/elps.201900109","type":"journal-article","created":{"date-parts":[[2019,9,19]],"date-time":"2019-09-19T06:06:15Z","timestamp":1568873175000},"page":"3057-3061","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of the elapsed time between sampling and formalin fixation on the <i>N<\/i>\u2010glycosylation profile of mouse tissue specimens"],"prefix":"10.1002","volume":"40","author":[{"given":"Boglarka","family":"Donczo","sequence":"first","affiliation":[{"name":"Horv\u00e1th Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabor","family":"Kiraly","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology University of Debrecen  Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1369-668X","authenticated-orcid":false,"given":"Andras","family":"Guttman","sequence":"additional","affiliation":[{"name":"Horv\u00e1th Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"MTA\u2010PE Translational Glycomics Research Group, Research Institute for Biomolecular and Chemical Engineering University of Pannonia  Veszprem Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08665.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.03.065"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2008.06.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011062223612"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05816"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.09.108"},{"key":"e_1_2_8_8_1","volume-title":"Essentials of Glycobiology","author":"Varki A.","year":"2009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2000.143"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006090"},{"key":"e_1_2_8_12_1","author":"Yoshida Y.","year":"2018","journal-title":"BioEssays"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414593112"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)15004-1"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq175"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2018.05.007"},{"key":"e_1_2_8_17_1","author":"Thi Sam N.","year":"2018","journal-title":"J. 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