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This study aims to develop a quantum dots (QDs)\u2010based nanoprobe Cyanine5\u2010DDDLEVLFQFPGLVPRGSGGHHHHHH\u2010QDs (Cy5\u2010LEVLVP\u2010QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5\u2010LEVLVP and QDs were allowed to bind with each other through metal affinity interaction and then injected the Cy5\u2010LEVLVP\u2010QD complex into a capillary with different bends, followed by related enzyme that can cleave the Cy5\u2010LEVLVP peptide. The fluorescence of Cy5 was excited by QDs due to F\u00f6rster resonance energy transfer. By monitoring the peaks produced by the original Cy5\u2010LEVLVP\u2010QD complex and a significant fluorescence change, sensitive analysis of two different enzymes was conducted. Therefore, the novel approach of using capillaries with semicircular bends could prove particularly useful for enzyme investigating in disease.<\/jats:p>","DOI":"10.1002\/elps.202000023","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T07:23:01Z","timestamp":1582528981000},"page":"1103-1108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multienzyme detection and in\u2010situ monitoring of enzyme activity by bending CE using quantum dots\u2010based polypeptide substrate"],"prefix":"10.1002","volume":"41","author":[{"given":"Lin","family":"Qiu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Engineering and Life Science Changzhou University  Changzhou Jiangsu P. R. 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