{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:44:37Z","timestamp":1760985877723,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM136334","U54GM105816"],"award-info":[{"award-number":["R35GM136334","U54GM105816"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stable heavy\u2010isotope labeling is commonly used in quantitative proteomics. Several common techniques incorporate deuterium (<jats:sup>2<\/jats:sup>H) as the heavy isotopic label using reductive amination with formaldehyde. Compared with alternatives, dimethyl labeling reagents are inexpensive and the labeling chemistry is simple and rapid. However, the substitution of hydrogen by deuterium can introduce subtle changes in peptides\u2019 polarities, leading to a shift in chromatographic retention times between deuterated and nondeuterated peptides that can lead to quantification deviations. Capillary zone electrophoresis has emerged as a complementary separation for ESI\u2013MS\u2010based proteomics, including targeted and quantitative approaches. The extent to which the deuterium isotope effect impacts CZE\u2010based proteomics, which separates peptides based on their S\/N ratios, has not been investigated. To address this issue, CZE was used to analyze dimethyl labeled <jats:italic>E. coli<\/jats:italic> tryptic digests in 100\u00a0min single\u2010shot analyses. The median migration time shift was 0.1\u00a0s for light versus heavy labeled peptides, which is 2.5% of the peak width. For comparison, nUHPLC\u2013ESI\u2013MS\/MS was used to analyze the same sample. In UPLC, deuterated peptides tended to elute earlier than nondeuterated peptides, with a retention shift of 3\u00a0s for light versus heavy labeled peptides, which is roughly half the peak width. This shift in separation time did not have a significant effect on quantitation for either method for equal mixing ratios of the light\u2010intermediate\u2010heavy isotope labeled samples.<\/jats:p>","DOI":"10.1002\/elps.202000051","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T02:26:40Z","timestamp":1590028000000},"page":"1374-1378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Minimal deuterium isotope effects in quantitation of dimethyl\u2010labeled complex proteomes analyzed with capillary zone electrophoresis\/mass spectrometry"],"prefix":"10.1002","volume":"41","author":[{"given":"Xiaojing","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Notre Dame  Notre Dame IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangliang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Notre Dame  Notre Dame IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1331-3191","authenticated-orcid":false,"given":"Norman J.","family":"Dovichi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Notre Dame  Notre Dame IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5927-5277","authenticated-orcid":false,"given":"Matthew M.","family":"Champion","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Notre Dame  Notre Dame IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/13690"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M400129-MCP200"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200025-MCP200"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0498563"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500061-MCP200"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.05.011"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio736"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.1.3.317"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.427"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr8006107"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac500140s"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0348625"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800297"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.21"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1344(199902)42:2<169::AID-JLCR178>3.0.CO;2-0"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac010583a"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma00182a040"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00135a034"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00169a022"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0704560"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900517"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.06.034"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4672"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b01147"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac991096m"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00100"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.09.035"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01388"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.202000051","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202000051","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.202000051","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202000051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:34:37Z","timestamp":1760524477000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202000051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,16]]},"references-count":29,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/elps.202000051"],"URL":"https:\/\/doi.org\/10.1002\/elps.202000051","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2020,6,16]]},"assertion":[{"value":"2019-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}