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Accumulating evidence suggests that low plasma levels of CA and its metabolites may serve as reliable diagnostic markers for early Parkinson's disease (PD) patients. In this study, we demonstrated a new MEKC method for determining CA and its three main downstream metabolites, paraxanthine (PX), theobromine (TB), and theophylline (TP), in human plasma. Plasma samples were collected, and analyzed using MEKC, after SPE. The running buffer was composed of 35\u00a0mM phosphate, pH of 10.5, and 25\u00a0mM SDS. The separation voltage was 15\u00a0kV and the detection wavelength was at 210\u00a0nm. Under the optimum conditions, four distinct analytes were completely separated and detected in less than 12\u00a0min. Method limits of detection were as low as 7.5\u00a0ng\/mL for CA, 5.0\u00a0ng\/mL for TB, and 4.0\u00a0ng\/mL for both PX and TP. The recoveries were between 88.0% and 105.9%. This method was successfully applied to 27 human plasma samples. The results indicate that the plasma concentrations of the four analytes are significantly lower in patients with early PD than in control subjects (<jats:italic>p<\/jats:italic> &lt; 0.05). The area under curve was improved to 0.839 when CA and its three main metabolites were included, suggesting that MEKC testing of CA, TP, TB, and PX may serve as a potential method for early diagnosis of PD.<\/jats:p>","DOI":"10.1002\/elps.202000059","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T10:17:10Z","timestamp":1591525030000},"page":"1392-1399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of caffeine and its main metabolites for early diagnosis of Parkinson's disease using micellar electrokinetic capillary chromatography"],"prefix":"10.1002","volume":"41","author":[{"given":"Yanzhen","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory of Environment and Human Health, School of Public Health  Hebei Medical University  Shijiazhuang P. R. 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