{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T10:59:56Z","timestamp":1789037996521,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"21-22","license":[{"start":{"date-parts":[[2020,6,21]],"date-time":"2020-06-21T00:00:00Z","timestamp":1592697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A quantification method for imatinib (IM), its major metabolite <jats:italic>N<\/jats:italic>\u2010desmethyl imatinib (NDI), and a degradation by\u2010product was developed using CE\u2013MS combined with an online concentration technique. The use of multiple reaction monitoring (MRM)\u2013MS\/MS further improved the sensitivity of this technology. Liquid\u2013liquid extraction (LLE) using tertiary butyl methyl ether yielded high recovery and reproducibility for the pretreatment of serum samples. The recovery rate exceeded 83% for all three analytes, and was 90% for IM. To improve quantification results, a conductivity\u2010induced online analyte concentration technique, field\u2010amplified sample stacking (FASS), was used. The S\/N ratios were improved at least 10\u2010fold when compared with conventional capillary zone electrophoresis. The detection limits were 0.2\u00a0ng\/mL for IM, 0.4\u00a0ng\/mL for NDI, and 4\u00a0ng\/mL for the degradation by\u2010product. These results are superior to those previously obtained by other reported methods. The new method was validated in terms of its selectivity, intra\u2010 and interday repeatability and accuracy, and sample storage stability, following the guidelines issued by the European Medicines Agency. Considering the convenient pretreatment procedure (LLE), superior sensitivity, and fast analysis speed (&lt;15\u00a0min), this method can be useful in the determination of imatinib levels in blood.<\/jats:p>","DOI":"10.1002\/elps.202000118","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T23:54:15Z","timestamp":1591919655000},"page":"1843-1850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Quantification of imatinib and related compounds using capillary electrophoresis\u2010tandem mass spectrometry with field\u2010amplified sample stacking"],"prefix":"10.1002","volume":"41","author":[{"given":"Tingting","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Chemistry the University of British Columbia  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry the University of British Columbia  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3669-6041","authenticated-orcid":false,"given":"David Da Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry the University of British Columbia  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2015.07.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2018.01.035"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022457"},{"key":"e_1_2_9_5_1","first-page":"935","volume":"8","author":"Cohen M. 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