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There are a variety of reasons that make their determination relevant, such as quality control, environment and food contamination assessment, drug\u2010release studies, among others. The lack of a chromophore makes aminoglycoside spectrophotometric detection particularly challenging, often requiring derivatization. In this work, an indirect detection method, making use of imidazole as a probe, applying CZE was successfully tested. It did not require derivatization, which simplified the sample preparation. Suitable figures of merit were obtained; recoveries between 95 and 105%, adequate repeatability and precision, correlation coefficients (<jats:italic>r<\/jats:italic>) above 0.998, and limits of detection (LODs) of 3.2 and 11\u00a0mg\/L for gentamicin and paromomycin, respectively. As a proof\u2010of\u2010concept, it was also applied in a simple controlled release experiment that was well fitted using the Hill equation.<\/jats:p>","DOI":"10.1002\/elps.202000160","type":"journal-article","created":{"date-parts":[[2020,7,19]],"date-time":"2020-07-19T15:19:51Z","timestamp":1595171991000},"page":"1576-1583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Derivatization\u2010free determination of aminoglycosides by CZE\u2013UV in pharmaceutical formulations"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1024-0089","authenticated-orcid":false,"given":"Tiago Augusto","family":"Donegatti","sequence":"first","affiliation":[{"name":"Departamento de F\u00edsica Qu\u00edmica e Matem\u00e1tica Universidade Federal de S\u00e3o Carlos \u2010 UFSCar  Sorocaba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4936-5647","authenticated-orcid":false,"given":"Alnilan","family":"Lobato","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Fundamental Instituto de Qu\u00edmica Universidade de S\u00e3o Paulo (USP)  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6327-7363","authenticated-orcid":false,"given":"Eliana A. 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