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We developed a dielectrophoretic corral trap in a microfluidic device that utilizes negative dielectrophoresis to capture single spherical polystyrene particles. Circular\u2010shaped micron\u2010size traps were employed inside the device and the three\u2010dimensional trap stiffness (restoring trapping force from equilibrium trapping location) was analyzed using 4.42\u00a0\u03bcm particles and 1\u00a0MHz of an alternating electric field from 6 V<jats:sub>P\u2010P<\/jats:sub> to 10 V<jats:sub>P\u2010P<\/jats:sub>. The trap stiffness increased exponentially in the <jats:italic>x<\/jats:italic>\u2010 and <jats:italic>y<\/jats:italic>\u2010direction, and linearly in the <jats:italic>z<\/jats:italic>\u2010direction. Image analysis of the trapped particle movements revealed that the trap stiffness is increased 608.4, 539.3, and 79.7% by increasing the voltage from 6 V<jats:sub>P\u2010P<\/jats:sub> to 10 V<jats:sub>P\u2010P<\/jats:sub> in the <jats:italic>x<\/jats:italic>\u2010, <jats:italic>y<\/jats:italic>\u2010, and <jats:italic>z<\/jats:italic>\u2010direction, respectively. The trap stiffness calculated from a finite element simulation of the device confirmed the experimental results. This analysis provides important insights to predict the trapping location, strength of the trapping, and optimum geometry for single particle trapping and its applications such as single\u2010molecule analysis and drug discovery.<\/jats:p>","DOI":"10.1002\/elps.202000222","type":"journal-article","created":{"date-parts":[[2020,12,19]],"date-time":"2020-12-19T07:33:40Z","timestamp":1608363220000},"page":"644-655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Quantitative analysis of the three\u2010dimensional trap stiffness of a dielectrophoretic corral trap"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4410-3285","authenticated-orcid":false,"given":"Mohammad Rizwen Ur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering University of Wisconsin\u2010Milwaukee  Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1669-1996","authenticated-orcid":false,"given":"Tae Joon","family":"Kwak","sequence":"additional","affiliation":[{"name":"Nancy E. and Peter C. 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