{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:40:34Z","timestamp":1760575234334,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"17-18","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enantioselectivity of the pharmacokinetics of methadone was investigated in anesthetized Shetland ponies after a single intravenous (0.5\u00a0mg\/kg methadone hydrochloride; <jats:italic>n<\/jats:italic> = 6) or constant rate infusion (0.25\u00a0mg\/kg bolus followed by 0.25\u00a0mg\/kg\/h methadone hydrochloride; <jats:italic>n<\/jats:italic> = 3) administration of racemic methadone. Plasma concentrations of <jats:sc>l<\/jats:sc>\u2010methadone and <jats:sc>d<\/jats:sc>\u2010methadone and their major metabolites, <jats:sc>l<\/jats:sc>\u2010 and <jats:sc>d<\/jats:sc>\u20102\u2010ethylidene\u20101,5\u2010dimethyl\u20103,3\u2010diphenylpyrrolidine (EDDP), respectively, were analyzed by CE with highly sulfated \u03b3\u2010cyclodextrin as chiral selector and electrokinetic analyte injection from liquid\/liquid extracts prepared at alkaline pH. In both trials, the <jats:sc>d<\/jats:sc>\u2010methadone concentrations were lower than those of <jats:sc>l<\/jats:sc>\u2010methadone and the <jats:sc>d<\/jats:sc>\u2010EDDP levels were lower than those of L\u2010EDDP. For the case of a single intravenous bolus injection, the plasma concentration versus time profile of methadone enantiomers was analyzed with a two\u2010compartment pharmacokinetic model. <jats:sc>l<\/jats:sc>\u2010methadone showed a slower elimination rate constant, a lower body clearance, and a smaller steady\u2010state volume of distribution than <jats:sc>d<\/jats:sc>\u2010methadone. <jats:sc>d<\/jats:sc>\u2010methadone and <jats:sc>d<\/jats:sc>\u2010EDDP were eliminated faster than their respective <jats:sc>l<\/jats:sc>\u2010enantiomers. This is the first study that outlines that the disposition of racemic methadone administered to anesthetized equines is enantioselective.<\/jats:p>","DOI":"10.1002\/elps.202100115","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T06:14:44Z","timestamp":1620800084000},"page":"1826-1831","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stereoselective methadone disposition after administration of racemic methadone to anesthetized Shetland ponies assessed by capillary electrophoresis"],"prefix":"10.1002","volume":"42","author":[{"given":"Friederike A.","family":"Sandbaumh\u00fcter","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Laboratory Institute for Infectious Diseases University of Bern  Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Gittel","sequence":"additional","affiliation":[{"name":"Department for Horses University of Leipzig  Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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