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For that, an amino acid\u2010based molecularly imprinted monolithic capillary column was designed and used as a stationary phase for selective separation of S\u2010citalopram (S\u2010CIT) for the first time. S\u2010CIT was selectively separated from the aqueous solution containing the other enantiomeric form of R\u2010CIT, which is the same in size and shape as the template molecule. Morphology of the molecularly imprinted (MIP S\u2010CIT) and non\u2010imprinted (NIP S\u2010CIT) monolithic capillary columns was observed by scanning electron microscopy. Imprinting efficiency of MIP S\u2010CIT monolithic capillary column used for selective S\u2010CIT separation was verified by comparing with NIP S\u2010CIT and calculated imprinting factor (I.F:1.81) proved the high selectivity of the MIP S\u2010CIT for S\u2010CIT. Cavities formed for S\u2010CIT form enabled selective (<jats:italic>\u03b1<\/jats:italic> = 2.08) separation of the target molecule from the other enantiomeric R\u2010CIT form. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 7.68 \u00d7 10<jats:sup>\u22126<\/jats:sup> m<jats:sup>2<\/jats:sup>\/Vs for R,S\u2010CIT at pH 7.0 10\u00a0mM PB and 50% ACN ratio. The performance of both MIP S\u2010CIT and NIP S\u2010CIT columns was estimated by repeating the R,S\u2010CIT separations with intra\u2010batch and inter\u2010batch studies for reproducibility of retention times of R,S\u2010CITs. Estimated RSD values that are lower than 2% suggest that the monolithic columns separate R,S\u2010CIT enantiomers without losing separation efficiency.<\/jats:p>","DOI":"10.1002\/elps.202100222","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T11:26:45Z","timestamp":1629286005000},"page":"2672-2682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["S\u2010citalopram imprinted monolithic columns for capillary electrochromatography enantioseparations"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4757-0161","authenticated-orcid":false,"given":"Ali","family":"Derazshamshir","sequence":"first","affiliation":[{"name":"Department of Chemistry Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7292-7241","authenticated-orcid":false,"given":"Ilg\u0131m","family":"G\u00f6kt\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3260-1639","authenticated-orcid":false,"given":"Fatma","family":"Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Chemistry Technology Division Bolu Abant Izzet Baysal University  Bolu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7548-5741","authenticated-orcid":false,"given":"Adil","family":"Denizli","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01990"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8044184"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.20178"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(99)00315-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00171-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.05.030"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.10.029"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.05.098"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.09.020"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4088\/PCC.v05n0202"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2017.061"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-12445-7_1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.115640"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22550"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.04.024"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.02.016"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2006.pto_295.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.54.432"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/0250-474X.29652"},{"key":"e_1_2_9_21_1","first-page":"513","volume":"5","author":"Serebruany V.","year":"2007","journal-title":"Clin. 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