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The device was fabricated in polymer films by rapid laser cutting and chemical bonding. After fabricating the prototype device, the separation performance of our device was evaluated using particles and cells. The effects of operational flow rate, cell diameter, and cell concentration on the separation performance were explored. Our device successfully separated tumor cells from polydisperse white blood cells according to their different migration modes and lateral positions. Then, the separation of rare cells was carried out using the high\u2010concentration lysed blood spiked with 200 tumor cells. Experimental results showed that 83.90% of the tumor cells could be recovered, while 99.87% of white blood cells could be removed. We successfully employed our device for processing clinical pleural effusion samples from patients with advanced metastatic breast cancer. Malignant tumor cells with an average purity of 2.37% could be effectively enriched, improving downstream diagnostic accuracy. Our device offers the advantages of label\u2010free operation, low cost, and fast fabrication, thus being a potential tool for effective cell separation.<\/jats:p>","DOI":"10.1002\/elps.202100232","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T21:41:06Z","timestamp":1633642866000},"page":"464-471","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Low\u2010cost polymer\u2010film spiral inertial microfluidic device for label\u2010free separation of malignant tumor cells"],"prefix":"10.1002","volume":"43","author":[{"given":"Cailian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Oncology Zhongda Hospital Southeast University  Nanjing P. R. 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