{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:49:31Z","timestamp":1790221771951,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9-10","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cardiac glycosides digoxin and digitoxin are used in therapy for the treatment of congestive heart failure. Moreover, these compounds can be responsible for intoxication cases caused by fortuitous ingestion of leaves of <jats:italic>Digitalis<\/jats:italic>. Due to the narrow therapeutic range of these drugs, therapeutic drug monitoring is recommended in the clinical practice. In this context, immunoassays\u2010based methods are generally employed but digoxin\u2010 and digitoxin\u2010like compounds can interfere with the analysis. The aim of this study was to develop and validate an original UPLC\u2013MS\/MS method for the determination of digoxin and digitoxin in plasma. The method shows adequate sensitivity and selectivity with acceptable matrix effects and very good linearity, accuracy, precision, and recovery. A simple liquid\u2013liquid extraction procedure was used for sample clean\u2010up. The method was applied for the analysis of <jats:italic>n<\/jats:italic> = 220 plasma samples collected in two different clinical chemistry laboratories and previously tested by the same immunoassay. The statistical comparison showed a relevant negative bias of the UPLC\u2013MS\/MS method versus the immunoassay. These results are consistent with an immunoassay overestimation of digoxin plasmatic levels due to cross\u2010reaction events with endogenous digoxin\u2010like substances.<\/jats:p>","DOI":"10.1002\/elps.202100290","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T02:54:00Z","timestamp":1644288840000},"page":"1019-1026","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Development of a new ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry method for the determination of digoxin and digitoxin in plasma: Comparison with a clinical immunoassay"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2176-650X","authenticated-orcid":false,"given":"Marco","family":"Ballotari","sequence":"first","affiliation":[{"name":"Unit of Forensic Medicine Department of Diagnostics and Public Health University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4728-6686","authenticated-orcid":false,"given":"Francesco","family":"Taus","sequence":"additional","affiliation":[{"name":"Unit of Forensic Medicine Department of Diagnostics and Public Health University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Tolle","sequence":"additional","affiliation":[{"name":"Unit of Forensic Medicine Department of Diagnostics and Public Health University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2454-0410","authenticated-orcid":false,"given":"Elisa","family":"Danese","sequence":"additional","affiliation":[{"name":"Section of Clinical Biochemistry University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romolo M.","family":"Dorizzi","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit Hub Laboratory Greater Area  Cesena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2187-9128","authenticated-orcid":false,"given":"Franco","family":"Tagliaro","sequence":"additional","affiliation":[{"name":"Unit of Forensic Medicine Department of Diagnostics and Public Health University of Verona  Verona Italy"},{"name":"World\u2010Class Research Center \u201cDigital Biodesign and Personalized Healthcare\u201d Sechenov First Moscow State Medical University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5997-1723","authenticated-orcid":false,"given":"Rossella","family":"Gottardo","sequence":"additional","affiliation":[{"name":"Unit of Forensic Medicine Department of Diagnostics and Public Health University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40256-018-0292-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejhf.584"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2001.00012.x"},{"key":"e_1_2_9_5_1","volume":"2019","author":"Negroni M. S.","year":"2019","journal-title":"CaseRep. Cardiol"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.21542\/gcsp.2021.2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jce.14894"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-72879-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.12221"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/2162-3619-1-4"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.109813"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.10.006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2165\/00139709-200625040-00007"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e31816b918f"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1309\/34BJECP7UK6FH13V"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.09.14"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.04.060"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.1653"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.05.026"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2009.09.025"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00462-8"},{"key":"e_1_2_9_22_1","unstructured":"International conference on harmonization of technical requirements for registration of pharmaceutical for human use. Complementary Guidelines on Methodology current step 4 version 2005."},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020361s"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2018-0075"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1781"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.11.023"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.988"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826070600757821"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.10.028"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/33.7.372"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.202100290","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:00:50Z","timestamp":1760526050000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202100290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,17]]},"references-count":29,"journal-issue":{"issue":"9-10","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/elps.202100290"],"URL":"https:\/\/doi.org\/10.1002\/elps.202100290","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,17]]},"assertion":[{"value":"2021-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}