{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T05:25:22Z","timestamp":1784093122194,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T00:00:00Z","timestamp":1679270400000},"content-version":"am","delay-in-days":328,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability to strategically induce or suppress cell lysis is critical for many cellular\u2010level diagnostic and therapeutic applications conducted within electrokinetic microfluidic platforms. The chemical and structural integrity of sub\u2010cellular components is important when inducing cell lysis. However, metal electrodes and electrolytes participate in undesirable electrochemical reactions that alter solution composition and potentially damage protein, RNA, and DNA integrity within device microenvironments. For many biomedical applications, cell viability must be maintained even when device\u2010imposed cell\u2010stressing stimuli (e.g., electrochemical reaction byproducts) are present. In this work, we explored a novel and tunable method to accurately induce or suppress device\u2010imposed artifacts on human red blood cell (RBC) lysis in non\u2010uniform AC electric fields. For precise tunability, a dielectric hafnium oxide (HfO<jats:sub>2<\/jats:sub>) layer was used to prevent electron transfer between the electrodes and the electric double layer and thus reduce harmful electrochemical reactions. Additionally, a low concentration of Triton X\u2010100 surfactant was explored as a tool to stabilize cell membrane integrity. The extent of hemolysis was studied as a function of time, electrode configuration (T\u2010shaped and star\u2010shaped), cell position, applied non\u2010uniform AC electric field, with uncoated and HfO<jats:sub>2<\/jats:sub> coated electrodes (50\u00a0nm), and absence and presence of Triton X\u2010100 (70\u00a0\u00b5M). Tangible outcomes include a parametric analysis relying upon literature and this work to design, tune, and operate electrokinetic microdevices to intentionally induce or suppress cellular lysis without altering intracellular components. Implications are that devices can be engineered to leverage or minimize device\u2010imposed biological artefacts extending the versatility and utility of electrokinetic diagnostics.<\/jats:p>","DOI":"10.1002\/elps.202100310","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T06:10:00Z","timestamp":1647756600000},"page":"1322-1336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Induction and suppression of cell lysis in an electrokinetic microfluidic system"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3736-0574","authenticated-orcid":false,"given":"Sanaz","family":"Habibi","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Michigan Technological University  Houghton Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwi Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Michigan Technological University  Houghton Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector","family":"Moncada\u2010Hernandez","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Michigan Technological University  Houghton Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2382-7831","authenticated-orcid":false,"given":"Adrienne R.","family":"Minerick","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Michigan Technological University  Houghton Michigan 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