{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:53:13Z","timestamp":1760575993642,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2022,9,4]],"date-time":"2022-09-04T00:00:00Z","timestamp":1662249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["STPGP 521545\u201018"],"award-info":[{"award-number":["STPGP 521545\u201018"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inorganic polyphosphates (polyPs) have been identified in eukaryotic and prokaryotic cells alike. Various extraction methods have been optimized as a necessary step before identification and measurement of these polymers. Three commercially available sodium polyP glasses were either dissolved or dissolved and extracted by two commonly used polyP extraction techniques \u2013 perchloric acid or buffered phenol\u2013chloroform. The products were separated by polyacrylamide gel electrophoresis (PAGE), stained with toluidine blue O, and the migration results quantitatively compared. Both extraction processes reduced the relative migration distances of the peak and leading edges, and the stained band lengths, suggesting reduced polyP migration and dispersion. <jats:sup>31<\/jats:sup>P diffusion\u2010ordered spectroscopy nuclear magnetic resonance confirmed that polyP extraction by perchloric acid or phenol\u2013chloroform processes reduced polyP diffusion coefficients and suggested hydrolytic degradation with stronger end\u2010chain signals. Reduced polyP diffusivity after extraction makes possible an overestimation of synthetic polyP chain length assignment when compared to unextracted polyP ladders with PAGE. The mechanism(s) for reduced synthetic polyP diffusion after extraction and intracellular chemical environment effects on migration are not known.<\/jats:p>","DOI":"10.1002\/elps.202100364","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T05:54:20Z","timestamp":1660715660000},"page":"2014-2022","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Extraction processes reduce polyphosphate ion migration, dispersion and diffusion as detected with gel electrophoresis and <sup>31<\/sup>P DOSY NMR"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1744-2708","authenticated-orcid":false,"given":"Lori","family":"Manoukian","sequence":"first","affiliation":[{"name":"Department of Mining and Materials Engineering McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"Correa","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Statistics McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin S.","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Chemistry McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Frigon","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9315-6984","authenticated-orcid":false,"given":"Sidney","family":"Omelon","sequence":"additional","affiliation":[{"name":"Department of Mining and Materials Engineering McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,4]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"5","volume-title":"Phosphorus in agriculture: global resources, trends and developments","author":"Smit AL","year":"2009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-9290.2008.00050.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/0470858192"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-41073-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.083007.093039"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5818"},{"issue":"3","key":"e_1_2_9_8_1","first-page":"139","article-title":"Oxidative stress protection by polyphosphate \u2013 new roles for an old player","volume":"176","author":"Gray MJ","year":"2019","journal-title":"Physiol Behav"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/es100191h"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.08.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05144"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01599a006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie50446a013"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01136a016"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01607a011"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.15698\/mic2017.01.551"},{"issue":"11","key":"e_1_2_9_17_1","first-page":"1211","article-title":"PPX1 gene overexpression has no influence on polyphosphates in Saccharomyces cerevisiae","volume":"79","author":"Lichko LP","year":"2014","journal-title":"Biochem"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2018.09.021"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2019.11.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN01031A"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201800227"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00567"},{"key":"e_1_2_9_23_1","first-page":"47","volume-title":"A guide to polyacrylamide gel electrophoresis and detection","author":"Bio\u2010Rad","year":"2012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800720"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00512-X"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90452-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600638"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1695690"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1486-2"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(87)90205-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.12.4966"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00588a006"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.49.5878"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/es071828p"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2011.06.020"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2019.101631"},{"volume-title":"Principles of polymer chemistry. The George Fisher Baker non\u2010resident lectureship in chemistry at Cornell University","year":"1953","author":"Flory PJ","key":"e_1_2_9_37_1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-024-0893-5"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/macp.201600436"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.202100364","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:41:37Z","timestamp":1760542897000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202100364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,4]]},"references-count":38,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/elps.202100364"],"URL":"https:\/\/doi.org\/10.1002\/elps.202100364","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2022,9,4]]},"assertion":[{"value":"2021-11-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}