{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T03:43:00Z","timestamp":1780544580842,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T00:00:00Z","timestamp":1650240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Determination of the broad\u2010spectrum antibiotics amoxicilline (AMX) and ceftazidime (CTZ) in blood serum and microdialysates of the subcutaneous tissue of the lower limbs is performed using CE with\u00a0contactless conductivity detection (C<jats:sup>4<\/jats:sup>D). Baseline separation of AMX is achieved in 0.5 M acetic acid as the background electrolyte and separation of CTZ in\u00a03.2 M acetic acid with addition of 13% v\/v methanol. The CE\u2010C<jats:sup>4<\/jats:sup>D determination is performed in a 25\u00a0\u00b5m capillary with suppression of the EOF using INST\u2010coating on an effective length of 18\u00a0cm and the attained migration time is 4.2\u00a0min for AMX and 4.4\u00a0min for CTZ. The analysis was performed using 20\u00a0\u00b5l of serum and 15\u00a0\u00b5l of microdialysate, treated by the addition of acetonitrile in a ratio of 1\/3 v\/v and the sample is injected into the capillary using the large volume sample stacking technique. The LOQ attained in the microdialysate is 148\u00a0ng\/ml for AMX and 339\u00a0ng\/ml for CTZ, and in serum 143\u00a0ng\/ml for AMX and 318\u00a0ng\/ml for CTZ. The CE\u2010C<jats:sup>4<\/jats:sup>D method is employed for monitoring the passage of AMX and CTZ from the blood circulatory system into the subcutaneous tissue at the sites of diabetic ulceration in patients suffering from diabetic foot syndrome and also for measuring the pharmacokinetics following intravenous application of bolus antibiotic doses.<\/jats:p>","DOI":"10.1002\/elps.202100366","type":"journal-article","created":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T05:23:32Z","timestamp":1643001812000},"page":"1129-1139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3489-9547","authenticated-orcid":false,"given":"Petr","family":"T\u016fma","sequence":"first","affiliation":[{"name":"Department of Hygiene Third Faculty of Medicine Charles University  Prague 10 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Ja\u010dek","sequence":"additional","affiliation":[{"name":"Department of Hygiene Third Faculty of Medicine Charles University  Prague 10 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanka","family":"Sommerov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Hygiene Third Faculty of Medicine Charles University  Prague 10 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Dlouh\u00fd","sequence":"additional","affiliation":[{"name":"Department of Hygiene Third Faculty of Medicine Charles University  Prague 10 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radka","family":"Jaro\u0161\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Centre Institute for Clinical and Experimental Medicine  Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitka","family":"Hus\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Centre Institute for Clinical and Experimental Medicine  Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Woskov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Centre Institute for Clinical and Experimental Medicine  Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladim\u00edra","family":"Fejfarov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Centre Institute for Clinical and Experimental Medicine  Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1002\/dmrr.3268","article-title":"Definitions and criteria for diabetic foot disease","volume":"36","author":"Netten JJ","year":"2020","journal-title":"Diabetes Metab Res Rev"},{"issue":"1","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","DOI":"10.1002\/dmrr.3269","article-title":"Guidelines on the prevention of foot ulcers in persons with diabetes (IWGDF 2019 update)","volume":"36","author":"Bus SA","year":"2020","journal-title":"Diabetes Metab Res Rev"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2356870"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2017.1281097"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2019.1676194"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.07.064"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.12.086"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900039"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300347"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.06.033"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461616"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.112906"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202000163"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.07.010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.09.032"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.05.014"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700478"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500819"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.05.044"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601213"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.09.076"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400044"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202100174"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201700440"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142301.ns0701s47"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.09.008"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.05.027"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121922"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201400585"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600167"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200206)23:11<1612::AID-ELPS1612>3.0.CO;2-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.05.050"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.05.049"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410185"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(02)00289-3"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.05.021"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.01.031"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.09.018"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.11.056"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5104"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.05.027"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.06.012"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2012.10.046"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.11.004"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.06.014"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100366","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elps.202100366","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202100366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:19:50Z","timestamp":1760530790000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202100366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,18]]},"references-count":45,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1002\/elps.202100366"],"URL":"https:\/\/doi.org\/10.1002\/elps.202100366","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,18]]},"assertion":[{"value":"2021-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}