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Its inhibitors can improve the expression of HIF and downstream genes, which can treat hypoxia\u2010related diseases. Therefore, the establishment of a reliable PHD2 inhibitors screening method is of great significance for the drug development of hypoxia\u2010related diseases. In this work, an accurate, rapid, and simple screening method for PHD2 inhibitors was introduced by capillary zone electrophoresis (CZE). In order to improve the detection sensitivity, the derivative reaction of \u03b1\u2010ketoglutaric acid (\u03b1\u2010OG) and 1,2\u2010diaminobenzene (OPD) was used to enhance the UV absorption of \u03b1\u2010OG (the substrate in the enzymatic reaction). The CZE method selected 20\u00a0mM Na<jats:sub>2<\/jats:sub>B<jats:sub>4<\/jats:sub>O<jats:sub>7<\/jats:sub> buffer (pH\u00a09.0) as the separation buffer, +25\u00a0kV as the separation voltage, 25\u00b0C\u00a0as the cartridge temperature, and 210\u00a0nm as the detection wavelength. Under this condition, the analysis of a single sample can be realized within 9\u00a0min. Compared with the existing reported methods, the present work can directly screen the PHD2 inhibitory activity of traditional Chinese medicine (TCM) extracts, which is of significance for the target\u2010purification of bioactive individual compounds from TCMs. Under the optimal conditions, the PHD2 inhibitor screening platform was successfully established, and it was found that 70% methanol\/water extracts of <jats:italic>Astragali Radix<\/jats:italic> and <jats:italic>Codonopsis pilosula<\/jats:italic> had good PHD2 inhibitory activity. Furthermore, the present work provides a novel approach for screening the PHD2 inhibitory activity of TCM extracts and the discovery of anti\u2010hypoxia bioactive compounds.<\/jats:p>","DOI":"10.1002\/elps.202200028","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T12:57:11Z","timestamp":1649681831000},"page":"1601-1610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE\u2010UV"],"prefix":"10.1002","volume":"43","author":[{"given":"Ying","family":"Zhang","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences (CAS)  Lanzhou P. R. China"},{"name":"University of Chinese Academy of Sciences  Beijing P. R. 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