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Up to now, there has been no specific DNA methylation\u2010based marker to distinguish skin cell DNA from other body fluids. The goal of this study is to develop a DNA methylation\u2010based assay to detect and identify skin cells collected at forensic crime scenes for use in DNA typing. For this reason, we have utilized a DNA methylation chip array\u2010based genome\u2010wide association study to identify skin\u2010specific DNA methylation markers. DNA obtained from skin along with other body fluids, such as semen, saliva, blood, and vaginal epithelia, were tested using five genes that were identified as sites for potential new epigenetic skin markers. Samples were collected, bisulfite converted, and subjected to real\u2010time polymerase chain reaction (PCR) with high\u2010resolution melt analysis. In our studies, when using WDR11, PON2, and NHSL1 assays with bisulfite\u2010modified PCR, skin\/sweat amplicons melted at lower temperatures compared to blood, saliva, semen, and vaginal epithelia. One\u2010way analysis of variance demonstrates that these three skin\/sweat markers are significantly different when compared with other body fluids (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). These results demonstrate that high\u2010resolution melt analysis is a promising technology to detect and identify skin\/sweat DNA from other body fluids.<\/jats:p>","DOI":"10.1002\/elps.202200120","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T11:25:11Z","timestamp":1670498711000},"page":"371-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["High\u2010resolution melt analysis for the detection of skin\/sweat via DNA methylation"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6908-938X","authenticated-orcid":false,"given":"Nicole","family":"Fernandez\u2010Tejero","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Florida International University  Miami Florida 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