{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:04:19Z","timestamp":1790456659911,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T00:00:00Z","timestamp":1656460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["19\u201018005Y"],"award-info":[{"award-number":["19\u201018005Y"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["SVV260560"],"award-info":[{"award-number":["SVV260560"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The feasibility of on\u2010capillary derivatization of saccharides by aromatic amine\u2010based fluorescent labeling agents was tested. To avoid the problematic evolution of gaseous hydrogen cyanide, the Schiff base reduction by sodium cyanoborohydride, as the second step of the standard reductive amination protocol, was omitted. Glucose was used as a model analyte and 7\u2010amino\u20101,3\u2010naphthalenedisulfonic acid as the labeling agent. Our experiments showed that the direct reaction of the saccharide with the labeling agent in 2.5\u2010M acetic acid yields a labeled product that is sufficiently stable to be separated from the labeling agent in 20\u2010mM phosphate buffer, pH 3.5, and detected using UV detection. The glucose and label zones were introduced separately into the capillary and mixed using a negative voltage. Mixing voltage, its duration, the concentration of acetic acid in the reaction zone, and the waiting time between mixing and separation were optimized. To show the applicability of the procedure to a broader range of analytes, a mixture of different types of saccharides, that is, xylose (pentose), fucose (hexose), glucose (hexose),\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylglucosamine (\n                    <jats:italic>N\u2010<\/jats:italic>\n                    acetylaminosaccharide), and lactose (disaccharide), was subjected to derivatization and analysis under the optimal conditions. The linearity and repeatability of the process were evaluated as critical parameters for its analytical applications. Six\u2010point calibration dependences in the 1\u201350\u00a0mM range showed excellent determination coefficients of 0.9992 or higher for all five saccharides tested. The repeatability of the labeled saccharide peak areas was between 2.2% and 4.3%.\n                  <\/jats:p>","DOI":"10.1002\/elps.202200136","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T05:09:08Z","timestamp":1655183348000},"page":"35-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["On\u2010capillary fluorescent labeling of saccharides for capillary electrophoresis"],"prefix":"10.1002","volume":"44","author":[{"given":"Kate\u0159ina","family":"\u010cokrtov\u00e1","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edt","family":"Mare\u0161","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1623-8844","authenticated-orcid":false,"given":"Tom\u00e1\u0161","family":"K\u0159\u00ed\u017eek","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/j.bbagen.2011.12.001","article-title":"Glycans as cancer biomarkers","volume":"1820","author":"Adamczyk B","year":"2012","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nrd1751","article-title":"Glycans in cancer and inflammation. 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