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The transmission of this protozoan by blood transfusion often results in high morbidity and mortality in recipients. A novel way to detect parasitized erythrocytes is by utilizing dielectrophoresis, an electrokinetic technique on a microfluidic platform, to improve the diagnostics of <jats:italic>Babesia<\/jats:italic> <jats:italic>spp<\/jats:italic>. The differences in the dielectric properties of <jats:italic>Babesia<\/jats:italic> <jats:italic>spp<\/jats:italic>.\u2013infected erythrocytes versus healthy erythrocytes were exploited to design a fast and cost\u2010effective diagnostic tool. One crucial factor for a successful diagnostic platform via dielectrophoretic separation is the dielectric characterization of <jats:italic>Babesia<\/jats:italic>\u2010infected erythrocytes, which is investigated in this paper. The influence of medium conductivity and erythrocytes phenotype and genotype over the first crossover frequency (<jats:italic>f<jats:sub>co1<\/jats:sub><\/jats:italic>) are used to quantify the dielectric properties of the infected cells. A sigmoidal curve was plotted via curve fitting of the single\u2010shell model, which has been proven appropriate for parasitized cell populations where considerable cell geometry variation occurs. The difference in these curves is relevant for the separation of cells population. Microliters of sample and reagent were used throughout this experiment; the scale, results obtained, and simplicity of the system often make it very suitable for point\u2010of\u2010care babesiosis disease diagnostics.<\/jats:p>","DOI":"10.1002\/elps.202200263","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T19:32:46Z","timestamp":1683747166000},"page":"988-1001","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Dielectric characterization of <i>Babesia bovis<\/i> using the dielectrophoretic crossover frequency"],"prefix":"10.1002","volume":"44","author":[{"given":"Raphael","family":"Oladokun","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomedical Engineering West Virginia University  Morgantown West Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezekiel","family":"Adekanmbi","sequence":"additional","affiliation":[{"name":"Intel Corporation  Chandler Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massaro","family":"Ueti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Washington State University  Pullman Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5003-7993","authenticated-orcid":false,"given":"Soumya","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomedical Engineering West Virginia University  Morgantown West Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,9]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Institute for International Cooperation in animal biologics","author":"Spickler AR","year":"2008"},{"key":"e_1_2_10_3_1","first-page":"2607","volume-title":"Principles of internal medicine","author":"Kasper DL","year":"2004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2007.03.002"},{"issue":"1","key":"e_1_2_10_5_1","first-page":"11","article-title":"Piroplasmosis in man; report of a case","volume":"9","author":"Skrabalo Z","year":"1957","journal-title":"Doc Med Geogr Trop"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1202018"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2006.00849.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050780"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035227"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.09.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05850.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)03111-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-013-0526-8"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10121563"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1996.03530450047031"},{"issue":"12","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.2460\/ajvr.1980.41.12.2102","article-title":"Canine babesiosis: indirect fluorescent antibody test for a North American isolate of Babesia gibsoni","volume":"41","author":"Anderson JF","year":"1980","journal-title":"Am J Vet Res"},{"key":"e_1_2_10_17_1","volume-title":"Recommendations for reducing the risk of transfusion\u2010transmitted babesiosis guidance for industry","author":"US Food and Drug Administration","year":"2019"},{"key":"e_1_2_10_18_1","unstructured":"Grifols Diagnostic Solutions I inventorProcleix Babesia Assay. 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