{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:57:41Z","timestamp":1760576261911,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"13-14","license":[{"start":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T00:00:00Z","timestamp":1679961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973450"],"award-info":[{"award-number":["81973450"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Elaphuri Davidiani Cornu (EDC) is the antler of the male P\u00e8re David's deer, which has been reported to have multiple biological activities, and its use as a traditional Chinese medicine (TCM) in China has been known for thousands of years. However, EDC is difficult to distinguish from other related species\u2013derived antlers in powder or extract form in TCM clinic use, such as Cervus elaphus Cornu (CEC) and Cervus nippon Cornu (CNC), both derived from Cervidae and easily confused with EDC. In this study, a strategy using peptidomics combined with mathematics set analysis was used to identify EDC\u2010specific peptide biomarkers, and four specific peptide biomarkers (Pep\u2010E1\u2013E4) were identified and validated. Pep\u2010E1, Pep\u2010E3, and Pep\u2010E4 could be exclusively detected in EDC samples, with relative peak areas of 0.298\u00a0\u00b1\u00a00.060, 0.039\u00a0\u00b1\u00a00.015, and 0.037\u00a0\u00b1\u00a00.008, whereas Pep\u2010E2 showed relative peak area of 0.516\u00a0\u00b1\u00a00.101 in EDC, 0.132\u00a0\u00b1\u00a00.026 in CEC, and 0.136\u00a0\u00b1\u00a00.047 in CNC samples, respectively. These four peptides are applicable to distinguish EDC from CEC and CNC, which is of great significance for the quality control of EDC.<\/jats:p>","DOI":"10.1002\/elps.202300023","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T01:34:27Z","timestamp":1679448867000},"page":"1088-1096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Discovery of Elaphuri Davidiani Cornu\u2013specific peptide biomarkers by peptidomics analysis\u2013based method"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9689-7283","authenticated-orcid":false,"given":"Rui","family":"Liu","sequence":"first","affiliation":[{"name":"National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under National Administration of Traditional Chinese Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"},{"name":"Animal\u2010Derived Chinese Medicine and Functional Peptides International Collaboration Joint Laboratory  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under National Administration of Traditional Chinese Medicine Nanjing University of Chinese Medicine  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Xiao","sequence":"additional","affiliation":[{"name":"National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under National Administration of Traditional Chinese Medicine Nanjing University of Chinese Medicine  Nanjing P. R. 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