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However, heterogeneity of drug releasing process and liposome itself challenged traditional pharmaceutical analytical techniques, especially <jats:italic>in vivo<\/jats:italic> pharmacokinetic studies. In this study, a novel liposomal doxorubicin (L\u2010DOX) pharmacokinetic analysis strategy was developed with capillary electrophoresis coupled with laser\u2010induced fluorescence (CE\u2010LIF) detector. The\u00a0background electrolyte (BGE) system was composed of borate and sodium dodecyl sulfate (SDS), which was optimized to successfully achieve simultaneous online separation and quantitative analysis of free DOX and liposome\u2010encapsulated DOX. The method was applied to the <jats:italic>in vivo<\/jats:italic> pharmacokinetic study of L\u2010DOX in rats. The results showed that the concentration of total DOX (T\u2010DOX) was gradually decreasing, while the concentration of L\u2010DOX was relatively stable, with a concentration of 31.6\u00a0\u00b1\u00a04.8 \u00b5g\/mL within 24\u00a0h. It was the first time to achieve liposomal drugs <jats:italic>in vivo<\/jats:italic> analysis with CE\u2010LIF. CE\u2010LIF was proved as potential rapidly real\u2010time analytical methods for liposomal drugs <jats:italic>in vivo<\/jats:italic> occurrence monitoring.<\/jats:p>","DOI":"10.1002\/elps.202300076","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T07:16:30Z","timestamp":1691997390000},"page":"1361-1368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liposomal doxorubicin and free doxorubicin <i>in vivo<\/i> quantitation method developed on CE\u2010LIF and its application in pharmacokinetic analysis"],"prefix":"10.1002","volume":"44","author":[{"given":"Cai","family":"Tie","sequence":"first","affiliation":[{"name":"State Key Laboratory Coal Resources and Safe Mining China University of Mining and Technology\u2010Beijing  Beijing P. R. China"},{"name":"School of Chemical and Environmental Engineering China University of Mining and Technology\u2010Beijing  Beijing P. R. 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