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Sample selection is a key factor, and femur and tooth have been traditionally recommended as the best substrate of genetic material. Recently, petrous bone (cochlear area) has been suggested as a better option due to its DNA yield. This research aims to evaluate the efficiency of petrous bone compared to other cranium samples (tooth) and postcranial long bones (femur and tibia). A total amount of 88 samples were selected from 38 different individuals. The samples were extracted by using an organic extraction protocol, DNA quantification by Quantifiler Trio kit and amplified with GlobalFiler kit. Results show that petrous bone outperforms other bone remains in quantification data, yielding 15\u201330 times more DNA than the others. DNA profile data presented likeness between petrous bone and tooth regarding detected alleles; however, the amount of DNA extracted in petrous bones allowed us to obtain more informative DNA profiles with superior quality. In conclusion, petrous bone or teeth sampling is recommended if DNA typing is going to be performed with environmentally degraded skeletal remains.<\/jats:p>","DOI":"10.1002\/elps.202300097","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T02:14:17Z","timestamp":1689819257000},"page":"1559-1568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A comparison between petrous bone and tooth, femur and tibia DNA analysis from degraded skeletal remains"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4419-4631","authenticated-orcid":false,"given":"Christian","family":"Haark\u00f6tter","sequence":"first","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana C.","family":"Vinueza\u2010Espinosa","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiomara","family":"G\u00e1lvez","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Saiz","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Isabel","family":"Medina\u2010Lozano","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9892-7386","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"Lorente","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Identification Department of Legal Medicine Toxicology and Physical Anthropology Faculty of Medicine University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/20961790.2018.1515594"},{"key":"e_1_2_9_3_1","first-page":"2852","article-title":"Time\u2010dependent effects of temperature and humidity on quantity of DNA in samples of human saliva, blood and semen in Kuwait","volume":"14","author":"Al\u2010Kandari NM","year":"2016","journal-title":"Int J Pharm Sci Res"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990101)20:8<1722::AID-ELPS1722>3.0.CO;2-4"},{"key":"e_1_2_9_5_1","volume-title":"The biophysical chemistry of nucleic acids and proteins","author":"Creighton TE","year":"2010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012567"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2021.102538"},{"key":"e_1_2_9_8_1","first-page":"486","article-title":"Success rates of nuclear short tandem repeat typing from different skeletal elements","volume":"48","author":"Milo\u0161 A","year":"2007","journal-title":"Croat Med J"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2022.102818"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigss.2009.09.029"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2017.12.023"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-44638-w"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2020.102305"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","unstructured":"IbrahimJ BrumfeldV AddadiY RubinS WeinerS BoarettoE et\u00a0al.The petrous bone contains high concentrations of osteocytes: one possible reason why ancient DNA is better preserved in this bone. 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