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To accelerate the optimization, native PAPC (n\u2010PAPC) standard was first analyzed by a commercial CE instrument with a photodiode array detector. The optimal separation buffer contained 60% (v\/v) acetonitrile, 40% (v\/v) methanol, 20\u00a0mM ammonium acetate, 0.5% (v\/v) formic acid, and 0.1% (v\/v) water. The selected separation voltage and capillary temperature were 20\u00a0kV and 23\u00b0C. The optimal CE separation buffer was then used for the low\u2010flow CE\u2010MS analysis. The selected MS conditions contained heated capillary temperature (250\u00b0C), capillary voltage (10\u00a0V), and injection time (1 s). No sheath gas was used for MS. The linear range for n\u2010PAPC was 2.5\u2013100.0\u00a0\u00b5g\/mL. The coefficient of determination (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>) was 0.9918. The concentration limit of detection was 1.52\u00a0\u00b5g\/mL, and the concentration limit of quantitation was 4.60\u00a0\u00b5g\/mL. The optimal low\u2010flow CE\u2010MS method showed good repeatability and sensitivity. The ox\u2010PAPC products in human HDLs were determined based on the <jats:italic>in vitro<\/jats:italic> ox\u2010PAPC products of n\u2010PAPC standard. Twenty\u2010one ox\u2010PAPC products have been analyzed in human HDLs. Uremic patients showed significantly higher levels of 15 ox\u2010PAPC products than healthy subjects.<\/jats:p>","DOI":"10.1002\/elps.202300139","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:57:17Z","timestamp":1700589437000},"page":"333-345","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Coupling capillary electrophoresis with mass spectrometry for the analysis of oxidized phospholipids in human high\u2010density lipoproteins"],"prefix":"10.1002","volume":"45","author":[{"given":"Chao\u2010Jung","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Integrated Medicine China Medical University  Taichung Taiwan"},{"name":"Department of Medical Research Proteomics Core Laboratory China Medical University Hospital  Taichung 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