{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T01:48:22Z","timestamp":1783129702607,"version":"3.54.6"},"reference-count":22,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hand, foot, and mouth disease is a serious public health problem, and the main pathogen is enterovirus 71 (EV71). Its capsid assembly mechanism including capsid protein processing has been widely studied. Full and empty capsids have different immunological efficacy. Therefore, tracking full\/empty capsid ratio throughout the EV71 production process is important to ensure consistent product quality and proper dosing response. The analysis of full\/empty capsid ratio of intact virus has been widely reported as well. A variety of techniques have been employed to evaluate the full\/empty capsid ratios. However, there has not been a rapid, reproducible, and robust assay to determine the full\/empty capsid ratios of final and in\u2010process products. In this study, a novel assay based on capillary zone electrophoresis was established. The separation of full and empty species could be achieved within 10\u00a0min and the ratio of peak areas was used to calculate the full\/empty capsid ratio directly. The results showed good reproducibility and linearity for the determination of full\/empty capsid ratios.<\/jats:p>","DOI":"10.1002\/elps.202300150","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T20:04:13Z","timestamp":1701115453000},"page":"327-332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of full and empty ratio of EV71 virus by using capillary zone electrophoresis"],"prefix":"10.1002","volume":"45","author":[{"given":"Jie\u2010Ru","family":"Wang","sequence":"first","affiliation":[{"name":"Sinovac Biotech Ltd.  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4537-0382","authenticated-orcid":false,"given":"Hong\u2010Xu","family":"Chen","sequence":"additional","affiliation":[{"name":"Sciex China  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua\u2010Qing","family":"Liu","sequence":"additional","affiliation":[{"name":"Sinovac Biotech Ltd.  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4101-3601","authenticated-orcid":false,"given":"Run","family":"Yu","sequence":"additional","affiliation":[{"name":"Sinovac Biotech Ltd.  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Jia","sequence":"additional","affiliation":[{"name":"Beijing BioCEart Technology Institute  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sinovac Biotech Ltd.  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Sinovac Biotech Ltd.  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"issue":"11","key":"e_1_2_9_2_1","first-page":"20652","article-title":"Analysis on the sequence of the whole genome of an isolated enterovirus 71 strain","volume":"8","author":"Gou E","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001066"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.12.9743-9752.1997"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218713"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2255"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103568"},{"issue":"11","key":"e_1_2_9_9_1","first-page":"1388","article-title":"Immune characteristics of two enterovirus 71 virion fractions","volume":"27","author":"Cheng C","year":"2014","journal-title":"Chin J Biol"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve234"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.202"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300946"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-0016(02)00019-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2019.09.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-006-0825-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-006-0003-y"},{"issue":"2","key":"e_1_2_9_17_1","first-page":"290","article-title":"Assessment of the suitability of a capillary zone electrophoresis method for determining isoform distribution of erythropoietin","volume":"11","author":"Bristow AF","year":"1999","journal-title":"Pharmeuropa"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600066"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008039200"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410075"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524020666200915105456"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-014-0087-3"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02070-12"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202300150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:38:18Z","timestamp":1760546298000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202300150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,27]]},"references-count":22,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/elps.202300150"],"URL":"https:\/\/doi.org\/10.1002\/elps.202300150","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,27]]},"assertion":[{"value":"2022-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}