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The impact of different dyes, including ethidium bromide, SYBR Green and SYBR Gold, was compared based on detection and separation of DNA plasmid topoisomers. Use of SYBR Gold enabled improvement of detection sensitivity by 15\u2010fold while maintaining good separation resolution of the different topoisomers. The baseline dropped with the use SYBR Gold but was overcome by the employment of a capillary with longer ineffective length (40 vs. 20\u00a0cm). Separation resolution and reproducibility were impacted by the concentration of SYBR Gold and hydroxypropyl methylcellulose. With the use of a short capillary (10\u00a0cm effective length and 50\u00a0cm total length), fast separations of supercoiled, linear, open circular, and other isoforms were accomplished within 8\u00a0min. Appropriate capillary cleaning with 0.1\u00a0M sodium hydroxide\/0.1\u00a0M hydrochloric acid and capillary storage with 0.1\u00a0M hydrochloric acid ensured good separation reproducibility of 217 runs during an extended period of use.<\/jats:p>","DOI":"10.1002\/elps.202300212","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T08:56:35Z","timestamp":1709110595000},"page":"1389-1397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sensitive and rapid analysis of plasmid DNA topology isoforms by capillary gel electrophoresis with laser induced fluorescence in uncoated capillary"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8527-1982","authenticated-orcid":false,"given":"Yan","family":"He","sequence":"first","affiliation":[{"name":"Analytical Research and Development Pfizer Inc  Chesterfield Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Orlet","sequence":"additional","affiliation":[{"name":"Analytical Research and Development Pfizer Inc  Chesterfield Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Cunio","sequence":"additional","affiliation":[{"name":"Analytical Research and Development Pfizer Inc  Chesterfield Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Thompson","sequence":"additional","affiliation":[{"name":"Analytical Research and Development Pfizer Inc  Chesterfield Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T","family":"Jones","sequence":"additional","affiliation":[{"name":"Analytical Research and Development Pfizer Inc  Chesterfield Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118870914.ch2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2009.02.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PLAS-0028-2014"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/blr.2007.9905"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh124"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.11.006"},{"key":"e_1_2_9_8_1","volume-title":"Molecular cloning: A laboratory manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_9_9_1","unstructured":"HauglandRP YueST MillardPJ all of Eugene RothBL.Corvallis all of Oreg.Cyclic substituted unsymetrical cyanine dye U. 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