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The inflammatory state caused by excessive accumulation of body fat affects the pathways related to the control of glycemic homeostasis, leading to a high demand for insulin, to subsequent failure of stressed \u03b2 cells, and development of type 2 diabetes mellitus (T2DM). The study of new endocrine signalers, such as bile acids (BAs), becomes necessary as it allows the development of alternatives for T2DM treatment. In this work, a methodology was developed to quantify tauroursodeoxycholic BA (TUDCA) in liver cells of the HepG2 strain treated in hyperlipidic medium. This BA helps to improve insulin clearance by increasing the expression of the insulin\u2010degrading enzyme, restoring sensitivity to this hormone, and making it viable for treating T2DM. Herein, a targeted metabolomic method for TUDCA determination in extracellular medium of hepatocyte matrices by micellar electrokinetic chromatography\u2010UV was optimized, validated, and applied. The optimized background electrolyte was composed of 40\u00a0mmol\/L sodium cholate and 30\u00a0mmol\/L sodium tetraborate at pH 9.0. The following figures of merit were evaluated: linearity, limit of quantification, limit of detection, accuracy, and precision. Data obtained with the validated electrophoretic method showed a self\u2010stimulation of TUDCA production in media supplemented only with BA. On the other hand, TUDCA concentration was reduced in the hyperlipidic medium. This suggests that, in these media, the effect of TUDCA is reduced, such as self\u2010stimulated production and consequent regulation of glycemic homeostasis. Therefore, the results reinforce the need for investigating TUDCA as a potential T2DM biomarker as well as its use to treat several comorbidities, such as obesity and diabetes mellitus.<\/jats:p>","DOI":"10.1002\/elps.202300223","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T06:59:11Z","timestamp":1716361151000},"page":"1252-1264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of tauroursodeoxycholic acid in liver cells\u2019 cultures by MEKC: Initial hints to comprehend its role in diabetes mellitus of obese individuals"],"prefix":"10.1002","volume":"45","author":[{"given":"Bruna Eduarda Santos","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"Laboratory of Analysis of Biomolecules Tiselius Institute of Chemistry Universidade Estadual de Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Roberta Rodrigues","family":"Muniz","sequence":"additional","affiliation":[{"name":"Institute of Biology Universidade Estadual de Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago dosReis","family":"Araujo","sequence":"additional","affiliation":[{"name":"Institute of Biology Universidade Estadual de Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Everardo","family":"Magalh\u00e3es Carneiro","sequence":"additional","affiliation":[{"name":"Institute of Biology Universidade Estadual de Campinas  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3899-2917","authenticated-orcid":false,"given":"Ana Val\u00e9ria Colnaghi","family":"Simionato","sequence":"additional","affiliation":[{"name":"Laboratory of Analysis of Biomolecules Tiselius Institute of Chemistry Universidade Estadual de Campinas  Campinas Brazil"},{"name":"National Institute of Science 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