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Grape\u2010specific proteins are more reliable markers than tartaric acid and other small organic acids (tartaric acid natural source are not only grape but also apple, mango, and other plants). The Yavne winery (located in the Central District of Israel) is stated to be the largest known wine production complex from the Byzantine period (ca. 1500 years ago). The site has been excavated recently, and a number of wine jar have been recovered. We have applied our ethylene vinyl acetate (EVA) (EVA studded with strong cation and anion exchangers) diskettes to the inner surface of a number of jars, thus capturing residual grape proteins therein. Via mass spectrometry analyses, we have been able to identify four grape and three yeast proteins. This has been possible because the EVA films, applied to such surfaces, are able to harvest and concentrate any trace species, rendering them amenable to instrumental analysis. Our analysis makes it possible to propose an explanation for the Holy Grail phenomenon as a dish in which wine or water begins to smell pleasant. We attribute this to the slow release of terpenes, aldehydes, and ketones from the clay walls of pottery. After digital modeling, we identified that \u201cscallop\u2010shaped\u201d niches in winery were used for the condensation of high percentage alcohol by passive evaporation from fermentation tanks.<\/jats:p>","DOI":"10.1002\/elps.202300249","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:08:25Z","timestamp":1705536505000},"page":"1443-1449","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Holy Grail possibly made from clay"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4200-6599","authenticated-orcid":false,"given":"Gleb","family":"Zilberstein","sequence":"first","affiliation":[{"name":"SpringStyle Tech Design Ltd  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Zilberstein","sequence":"additional","affiliation":[{"name":"SpringStyle Tech Design Ltd  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pier Giorgio","family":"Righetti","sequence":"additional","affiliation":[{"name":"Department of Chemistry Materials and Chemical Engineering \u201cGiulio Natta \u201d  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106406"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20548923.2020.1738728"},{"key":"e_1_2_9_4_1","first-page":"169","article-title":"The use of Ascalon wine in the medical writers of the fourth to the seventh centuries","volume":"43","author":"Mayerson P","year":"1993","journal-title":"Israel Explor J"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2019.1624164"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00080"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2021.104113"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.05.010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.08.010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr200266w"},{"key":"e_1_2_9_11_1","first-page":"253","article-title":"In taberna quando sumus: a drunkard's cakewalk through wine proteomics","volume":"50","author":"Righetti PG","year":"2012","journal-title":"Food Technol Biotechnol"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.02.023"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jas.2023.105906"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/heritage4040188"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0266085"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ces2.10073"},{"volume-title":"The wine bible","year":"2015","author":"MacNeil K","key":"e_1_2_9_17_1"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-6255-6"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.202300249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:24:30Z","timestamp":1760531070000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.202300249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,17]]},"references-count":17,"journal-issue":{"issue":"15-16","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/elps.202300249"],"URL":"https:\/\/doi.org\/10.1002\/elps.202300249","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2024,1,17]]},"assertion":[{"value":"2023-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}