{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:35:20Z","timestamp":1760574920327,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"23-24","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lymphocyte activation is critical in regulating immune responses. The resulting T\u2010cell proliferation has been implicated in the pathogenesis of a variety of autoimmune diseases, such as SLE and rheumatoid arthritis. ConA (concanavalin A)\u2010induced activation has been widely used in the T lymphocytes model of immune\u2010mediated liver injury, autoimmune hepatitis, and so on. In those works, it usually requires fluorescent labeling or cell staining to confirm whether the cells are transformed successfully after medicine treatment to figure out efficacy\/pharmacology. The detection preparation steps are time\u2010consuming and have limitations for further proteomic\/genomic identifications. Here, a label\u2010free microfluidic method is established to detect lymphocyte activation degree. The lymphocyte and ConA\u2010activated lymphocyte were investigated by a microfluidic device. According to where single cells in the sample were captured in the designed channel, lymphocyte and ConA\u2010activated samples are differentiated and characterized by population electric field factors, 2.08\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> and 2.21\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\u00a0V\/m, respectively. Furthermore, salidroside, a herbal medicine that was documented to promote the transformation, was used to treat lymphocyte cells, and the treated cell population is detected to be 2.67\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\u00a0V\/m. The characterization indicates an increasing trend with the activation degree. The result maintains a high consistency with traditional staining methods with transformed cells of 15.8%, 28.8%, and 48.3% in each cell population. Dielectrophoresis is promising to work as a tool for detecting lymphocyte transformation and medical efficacy detection.<\/jats:p>","DOI":"10.1002\/elps.202400060","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T05:01:45Z","timestamp":1723179705000},"page":"2125-2131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Label\u2010free detection of ConA\u2010induced T\u2010lymphocyte activation at single\u2010cell level by microfluidics"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-443X","authenticated-orcid":false,"given":"Yameng","family":"Liu","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohu","family":"Wang","sequence":"additional","affiliation":[{"name":"Intelligent 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