{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:06:26Z","timestamp":1771243586197,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100011033","name":"Thermo Fisher Scientific","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CBET #1605351"],"award-info":[{"award-number":["CBET #1605351"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We demonstrate a gel\u2010free electrophoretic separation of Sanger sequencing fragments up to 782 bases in length using nonionic wormlike micelles as drag\u2010tags in micelle\u2010tagging electrophoresis (MTE). This is an increase of 280 bases over previous MTE methods and a nearly three\u2010fold improvement over end\u2010labeled free\u2010solution electrophoresis (ELFSE) methods that use covalently attached drag\u2010tags. For MTE, C18 alkane groups are attached to primers prior to their enzymatic extension. This alkane group provides a binding site for wormlike micelles composed of CiEj\u2010type nonionic surfactants in the running buffer. Transient attachment of micelles to the C18 alkane group provides a highly uniform drag, equal to that of an ssDNA fragment 309 bases long. To account for slight mobility differences among the BigDye chain terminators, we developed a two\u2010parameter time\u2010shifting procedure to align the electropherograms for each termination chemistry. The increase in read length for this low\u2010viscosity buffer (2.1\u00a0cP) is attributed to the alignment procedure, the large yet uniform drag, and the small degree of adsorption\u2010based band broadening.<\/jats:p>","DOI":"10.1002\/elps.70047","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T04:34:38Z","timestamp":1761107678000},"page":"5-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long\u2010Read, High\u2010Resolution Sanger Sequencing by Micelle\u2010Tagging Electrophoresis"],"prefix":"10.1002","volume":"47","author":[{"given":"Randall","family":"Gamble","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Carnegie Mellon University  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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