{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T19:30:51Z","timestamp":1785007851988,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Brucellosis is a neglected zoonotic disease that continues to impact global public health and livestock economies, particularly in regions with limited diagnostic infrastructure. Its causative agent,\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    , is difficult to detect due to its intracellular lifestyle and the nonspecific symptoms it causes in humans. This study demonstrates the experimental application of dielectrophoresis (DEP) in a microfluidic device for the selective manipulation of polystyrene beads and inactivated\n                    <jats:italic>B. abortus<\/jats:italic>\n                    bacteria. By tuning the frequency and medium conductivity, reliable combined negative dielectrophoretic (nDEP) and hydrodynamic flow responses were achieved, leading to the deflection of bacterial cells across the microchannel within a critical vertical window for particle control. Distinct particle trajectories were observed under varying electric field conditions, confirming effective separation without the need for labels or biochemical markers, except for visual validation. This label\u2010free strategy enables rapid sample processing and has the potential to be integrated into portable platforms for on\u2010site diagnostics. The results highlight the feasibility of DEP\u2010based approaches for pathogen separation and support their future implementation in brucellosis surveillance and point\u2010of\u2010care testing.\n                  <\/jats:p>","DOI":"10.1002\/elps.70055","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T11:33:51Z","timestamp":1763033631000},"page":"68-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microfluidic Dielectrophoretic Platform for the Manipulation of\n                    <i>Brucella abortus<\/i>\n                    Bacteria: Toward Rapid Diagnostic Solutions"],"prefix":"10.1002","volume":"47","author":[{"given":"Katherine","family":"Acu\u00f1a\u2010Uma\u00f1a","sequence":"first","affiliation":[{"name":"Medical Device Engineering Master's Program Instituto Tecnol\u00f3gico de Costa Rica (ITCR)  Cartago Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefany","family":"Garc\u00eda\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"School of Physics Universidad De Costa Rica (UCR)  San Jos\u00e9 Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Mairena\u2010Salazar","sequence":"additional","affiliation":[{"name":"School of Physics Universidad De Costa Rica (UCR)  San Jos\u00e9 Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazareth","family":"Ruiz\u2010Villalobos","sequence":"additional","affiliation":[{"name":"Programa De Investigaci\u00f3n en Enfermedades Tropicales Escuela De Medicina Veterinaria Universidad Nacional  Heredia Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Guzm\u00e1n\u2010Verri","sequence":"additional","affiliation":[{"name":"Programa De Investigaci\u00f3n en Enfermedades Tropicales Escuela De Medicina Veterinaria Universidad Nacional  Heredia Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Torres\u2010Castro","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Stony Brook University  Stony Brook New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Lesser\u2010Rojas","sequence":"additional","affiliation":[{"name":"School of Physics Universidad De Costa Rica (UCR)  San Jos\u00e9 Costa Rica"},{"name":"Research Center in Atomic Nuclear and Molecular Sciences (CICANUM) Universidad De Costa Rica (UCR)  San Jos\u00e9 Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259\u2010024\u201010606\u20107"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2909.230052"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182380"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13182955"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974\u20100147.2020.00002.1"},{"key":"e_1_2_9_7_1","unstructured":"World Organization for Animal Health \"Brucellosis \"(2025) https:\/\/www.woah.org\/en\/disease\/brucellosis\/."},{"key":"e_1_2_9_8_1","unstructured":"M. J.Corbel \u201cBrucellosis in humans and animals Food and Agriculture Organization of the United Nations \u201dWorld Health Organization and World Organization for Animal Health (2006) https:\/\/iris.who.int\/handle\/10665\/43597."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010018\u20100295\u20103"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207749"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2021.08.073"},{"key":"e_1_2_9_12_1","unstructured":"Y.Wu \u201cDiscrete Particle Dynamics Models: Computational Aspects and Applications\u201d (doctoral dissertations and master's theses Embry\u2010Riddle Aeronautical University 2024)."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202200055"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi14112117"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202000156"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010019\u201002308\u2010w"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac049863r"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/b819054b"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4795856"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.5049148"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24087077"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/w13121678"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1lc20307j"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201900340"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40430\u2010023\u201004523\u20100"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192439399"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010020\u201002467\u20101"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202100304"},{"key":"e_1_2_9_29_1","doi-asserted-by":"crossref","unstructured":"M. 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