{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:45:07Z","timestamp":1777869907307,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2023\u2010PT350\u201001"],"award-info":[{"award-number":["2023\u2010PT350\u201001"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recombinant adeno\u2010associated viruses (AAVs) have become a leading platform for in vivo gene therapy. The AAV capsid is assembled from 60 individual protein subunits derived from three overlapping viral proteins (VPs): VP1, VP2, and VP3. The VP's ratio distribution affects the capsid trafficking and transgene expression. Quantitative analysis of the ratio is important in monitoring the potency of the AAVs. Sodium dodecyl sulfate capillary electrophoresis (CE\u2010SDS) offers automated and quantitative determination of the VP ratio distribution, and the method has been widely used in the application. The CE\u2010SDS method described was designed to ensure the consistent determination of AAV VP ratios across various sample concentrations. UV\u2010absorption detection was chosen for this purpose as it negated the need for any labeling in the process. To compensate for the lower sensitivity associated with UV\u2010absorption detection, methanol precipitation was employed during sample preparation, and a stacking injection technique was optimized for the samples in the study under CE conditions. These two techniques were found to be highly compatible. The method demonstrated robust linearity for AAV serotype 9 concentrations and exhibited good multiday precision. The VP ratios were successfully determined in AAV9 samples across a concentration range of 6.15\u00a0\u00d7\u00a010\n                    <jats:sup>12<\/jats:sup>\n                    to 1.85\u00a0\u00d7\u00a010\n                    <jats:sup>13<\/jats:sup>\n                    \u00a0GC\/mL. The relative standard deviation (RSD%) for VP ratio determination within this concentration range was maintained below 7% for VP3, VP2, and VP1. Additionally, this method was further applied to other AAV serotypes, including AAV2 and AAV8, illustrating its versatility and broad applicability.\n                  <\/jats:p>","DOI":"10.1002\/elps.70073","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:25:42Z","timestamp":1770881142000},"page":"243-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of AAV Capsid Protein Ratios by Sodium Dodecyl Sulfate Capillary Electrophoresis (CE\u2010SDS)\u2014UV Absorption Method"],"prefix":"10.1002","volume":"47","author":[{"given":"Yiran","family":"Li","sequence":"first","affiliation":[{"name":"National Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufei","family":"Sun","sequence":"additional","affiliation":[{"name":"Hubei University of Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Bi","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dening","family":"Pei","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihao","family":"Fu","sequence":"additional","affiliation":[{"name":"National Institutes 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Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control  Beijing China"},{"name":"State Key Laboratory of Drug Regulatory Science  Beijing 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Z.Peng E.Miller andM.Nilsson PerkinElmer Poster Presentation(2020) https:\/\/www.mabdesign.fr\/wp\u2010content\/uploads\/2020\/09\/Characterization\u2010of\u2010AAV\u2010structure.pdf."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010023\u201005097\u20105"},{"key":"e_1_2_8_16_1","unstructured":"T.Li M.Santos andA.Guttman Ultrahigh Sensitivity Analysis of Adeno\u2010Associated Virus Chromatography Today 2021; Buyers\u2019 Guide https:\/\/www.labmate\u2010online.com\/article\/bioanalytical\/40\/sciex\/ultrahigh\u2010sensitivity\u2010analysis\u2010of\u2010adeno\u2010associated\u2010virus\u2010aavbr\u2010capsid\u2010proteins\u2010by\u2010sodium\u2010dodecyl\u2010sulphate\u2010capillary\u2010gel\u2010electrophoresis\/2852."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.16155\/j.0254\u22121793.2023.11.04"},{"key":"e_1_2_8_18_1","unstructured":"T.Li M.Malik H.Yowanto andS.Mollah Purity Analysis of Adeno\u2010Associated Virus (AAV) Capsid Proteins Using CE\u2010SDS Method Sciex Application Note RUO\u2010MKT\u201002\u20109761\u2010A (SCIEX 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